| Field | Specification |
|---|---|
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
Cell cycle progression through the G1, S, G2, and M phases is tightly coordinated to ensure faithful DNA replication and division. A key mechanism of phase control is regulated protein degradation: the licensing factor CDT1 accumulates in G1 and is degraded as cells enter S phase, while Geminin accumulates during S, G2, and M and is degraded at the end of mitosis. The fluorescent ubiquitination-based cell cycle indicator (FUCCI) exploits these reciprocal oscillations by fusing fluorescent proteins to fragments of CDT1 and Geminin, allowing real-time visualization of cell cycle phase in living cells. This approach is widely used in studies of proliferation, differentiation, stem cell biology, and cancer.
Product Description & Applications
The Cell Cycle Reporter Lentivirus delivers a FUCCI-based system for visualizing cell cycle progression in living cells without fixation. Human CDT1 (amino acids 30-120), which accumulates in G1, is fused to RFP, and human Geminin (amino acids 1-110), which accumulates in G2/M, is fused to GFP. A CMV promoter drives RFP-CDT1, GFP-Geminin, and a drug selection marker as a single transcript, with self-cleaving T2A and P2A sequences yielding three separate proteins for balanced reporter expression.
This arrangement enables precise monitoring of all cell cycle phases in stable or transiently transduced cells. Supplied as VSV-G-pseudotyped particles purified by PEG precipitation and sucrose gradient centrifugation, the product suits a wide range of mammalian cells, including primary, thawed, and pluripotent stem cells.
About This Product
This reporter lentivirus places a EGFP, GFP, hCDT1-RFP + hGemn-GFP, Luc, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the Cell cycle (phases) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
Glioblastoma invasion into different organoid hosts reveals cell-intrinsic and proliferative migratory programs.
Akhunbay-Fudge CY, Irving BK, Ismail A, Samuel S, et al.
iScience, 2026. DOI: 10.1016/j.isci.2026.115361
Product(s) used: LTV-0052
Usage: FUCCI Cell Cycle Reporter Lentivirus (LTV-0052) used to generate stable GFP/RFP cell-cycle reporter glioblastoma cell lines for live-cell tracking of invasion dynamics into cerebral organoid hosts.
View article →A quantitative characterization of the heterogeneous response of glioblastoma U-87 MG cell line to temozolomide.
Dixit P, Djafer-Cherif I, Shah S, Drabik K, et al.
Scientific Reports, 2025. DOI: 10.1038/s41598-025-99426-6
Product(s) used: LTV-0052
Usage: FUCCI Cell Cycle Reporter Lentivirus (LTV-0052-1S) transduced into U-87 MG glioblastoma cells; puromycin-selected stable FUCCI cells used to quantify cell-cycle phase heterogeneity and differential temozolomide sensitivity by live fluorescence microscopy.
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