| Field | Specification |
|---|---|
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
FOXC2 belongs to the Forkhead box family of transcription factors, which share a conserved DNA-binding domain but differ in flanking sequences that determine target specificity. FOXC2 controls the expression of genes involved in normal development, including the formation of veins, lymphatic vessels, lungs, eyes, kidneys, the urinary tract, and the cardiovascular system. It is a key regulator of lymphatic vascular development and immune cell transport. FOXC2 is also linked to epithelial-mesenchymal transition and is studied in the context of cancer progression and metastasis, making it relevant to developmental biology research.
Product Description & Applications
The FOXC2 Reporter Lentivirus is a transcription factor reporter system built with tandem repeats of FOXC2 DNA-binding elements designed to preferentially read out FOXC2 activity over other Forkhead transcription factors. The elements drive a reporter gene downstream of a minimal promoter, giving a sensitive fluorescent or luminescent readout of FOXC2 transcriptional activity and the signaling pathways that activate it. Reporter options include EGFP, GFP, mCherry, RFP, d2GFP, and firefly luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.
It supports stable reporter cell line generation for studying FOXC2 activity in development and disease.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, mCherry, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the FOXC2 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
Intracellular pH dynamics respond to extracellular matrix stiffening and mediate vasculogenic mimicry through β-catenin.
Lund LM, Marchi AN, Alderfer L, Hall E, et al.
Cell Death & Disease, 2025. DOI: 10.1038/s41419-025-08014-z
Product(s) used: LTV-0061
Usage: FOXC2 Reporter Lentivirus (FOXC2-TAG-Puro, LipExoGen) used to establish single-cell transcriptional reporters; GFP signal demonstrated ECM stiffening activates FOXC2 to drive vasculogenic mimicry via intracellular pH/β-catenin axis.
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