| Field | Specification |
|---|---|
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
FOXF1 is a member of the Forkhead box family of transcription factors with essential roles in mesenchymal development, particularly of the lung, gut, and vasculature. It cooperates with other transcription factors to regulate tissue patterning and is required for normal lung morphogenesis. FOXF1 acts with ETS factors and SMAD4 to drive expression of genes such as ACVRL1. FOXF1 has been implicated in cancer progression and metastasis of lung and breast cancers and is required for the oncogenic properties of the PAX3-FOXO1 fusion in rhabdomyosarcoma, making it relevant to developmental and cancer research.
Product Description & Applications
The FOXF1 Reporter Lentivirus is a transcription factor reporter system built with tandem repeats of FOXF1/Activin Responsive Elements (FARE), which contain FOXF1, ETS, and SMAD4 DNA-binding elements derived from the ACVRL1 promoter. The elements drive a reporter gene downstream of a minimal promoter, giving a sensitive fluorescent or luminescent readout of FOXF1 transcriptional activity. A wide range of reporters is available, including EGFP, mCherry, BFP2, d2GFP, GFP, RFP, firefly luciferase, Gaussia luciferase, and Renilla luciferase, with selection markers including blasticidin, hygromycin, puromycin, and zeocin. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells.
It supports stable reporter cell line generation for studying FOXF1 activity in development and cancer.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXF1/FARE transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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