| Field | Specification |
|---|---|
| Product Type | |
| Reporter | |
| Selection Marker | Hygromycin, Zeocin, Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
FOXH1 (forkhead box H1) is a forkhead transcription factor and a key mediator of TGF-beta and Activin/Nodal signaling. Upon pathway activation, FOXH1 forms a transcriptionally active complex with SMAD2 and SMAD4 that binds a TGF-beta/Activin response element (TARE), originally identified in the goosecoid promoter. Through this complex, FOXH1 regulates mesendoderm differentiation genes during early embryonic development and contributes to anterior heart field development by inducing Mef2c and interacting with Nkx2-5. FOXH1 has also been implicated in the progression of epithelial carcinomas, including lung, breast, and colon cancers, through crosstalk with other signaling pathways, making it relevant to both developmental biology and oncology research.
Product Description & Applications
The FOXH1 Reporter Lentivirus places a reporter gene under the control of tandem goosecoid promoter-derived TGF-beta/Activin response elements (TARE), providing a sensitive readout of FOXH1 transcriptional activity in human and mouse cells. A broad range of reporter options is available, including fluorescent (GFP, EGFP, d2GFP, BFP2, mCherry, RFP), luminescent (firefly, Renilla, and Gaussia luciferase), and combined formats, with hygromycin, zeocin, puromycin, or blasticidin selection.
The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring TGF-beta and Activin/Nodal signaling, studying mesendoderm differentiation, and screening pathway modulators.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXH1/TARE transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Hygromycin, Zeocin, Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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