FOXQ1 Reporter Lentivirus

Background

FOXQ1 is a member of the forkhead box (FOX) family of winged-helix transcription factors, which bind forkhead DNA elements to regulate development, differentiation, and tissue homeostasis. FOXQ1 is a relatively weak transcriptional activator on its own, but in cancer its activity can be amplified through cooperation with oncogenic partners, promoting epithelial-to-mesenchymal transition, tumor progression, and metastasis. One notable partner is EWS-FLI1, a chimeric transcription factor produced by EWSR1 chromosomal translocations and the defining driver of Ewing sarcoma, an aggressive bone and soft-tissue cancer of children and adolescents. EWS-FLI1 binds GGAA microsatellites in enhancers and promoters, and FOXQ1 has been identified as a cooperative partner in this oncogenic transcriptional program.

Product Description & Applications

The FOXQ1 Reporter Lentivirus is a lentiviral transcription-factor reporter system that provides a sensitive fluorescent or luminescent readout of FOXQ1 transcriptional activity in mammalian cells. The construct contains tandem repeats of a composite sequence combining consensus FOXQ1 DNA-binding elements with GGAA microsatellites, enabling readout of the synergistic activities of FOXQ1 and the Ewing sarcoma fusion protein EWS-FLI1. The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cultures. A constitutively expressed selection marker (blasticidin, hygromycin, puromycin, or zeocin) supports stable reporter cell line generation. The system supports readout by fluorescence microscopy, flow cytometry, or luminometry for cancer transcription studies and drug discovery targeting EWS-FLI1-driven programs.

About This Product

This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXQ1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Hygromycin, Zeocin, Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.

Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.