| Field | Specification |
|---|---|
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
HOXB13 (homeobox B13) is a homeodomain transcription factor of the HOX family, regulators of body patterning and tissue identity during development. HOXB13 contributes to the development and homeostasis of posterior structures and hormone-responsive tissues. In the prostate it acts as a context-dependent modulator of androgen receptor signaling, including functions as a repressor of androgen receptor activity, and thereby influences epithelial differentiation and growth regulation. Germline variants in HOXB13 are associated with hereditary prostate cancer risk, and altered HOXB13 expression affects tumor cell behavior. The factor is studied for its roles in developmental patterning, hormone-responsive transcription, and prostate biology.
Product Description & Applications
The HOXB13 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of HOXB13 activity in mammalian cells. The construct contains tandem repeats of the HOXB13 DNA-binding element placed upstream of a minimal promoter driving the reporter gene, with an optimized upstream enhancer that maximizes signal-to-noise. A constitutive drug selection marker (Blasticidin or Puromycin) enables generation of stable polyclonal reporter cell lines. Stable lentiviral integration provides consistent reporter expression suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, the product is well suited to studying HOXB13 transcriptional activity in primary and difficult-to-transfect cells for developmental and endocrine research.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the Androgen Receptor (AR) Pathway (HOXB13) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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