| Field | Specification |
|---|---|
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
KLF5 (Krüppel-like factor 5) is a zinc-finger transcription factor that binds GC-rich cis-elements to regulate cell growth, proliferation, differentiation, and embryonic development. KLF5 and the related factor KLF4 recognize similar but non-identical GC-rich sequences and often act as opposing yin-yang regulators of gene expression. KLF5 mediates signaling in epithelial homeostasis and intestinal development, and its expression is frequently abnormal in human cancers and in vascular smooth muscle cells associated with cardiovascular disease. Because aberrant KLF5 activity contributes to tumor progression and vascular pathology, it has been proposed as a potential diagnostic biomarker and therapeutic target for cancer and cardiovascular disease.
Product Description & Applications
The KLF5 Reporter Lentivirus is a lentiviral transcription-factor reporter system that provides a sensitive fluorescent or luminescent readout of KLF5 transcriptional activity in mammalian cells. The construct contains tandem repeats of the gut-enriched Krüppel-like factor element and a KLF5 DNA-binding site derived from the human IGF1 promoter, driving reporter expression in response to KLF5. The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cultures. A constitutively expressed selection marker (blasticidin, hygromycin, puromycin, or zeocin) supports stable reporter cell line generation, and the broad choice of fluorescent and luminescent reporters supports readout by fluorescence microscopy, flow cytometry, or luminometry for studies of proliferation, differentiation, cancer, and cardiovascular biology.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the KLF5 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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