| Field | Specification |
|---|---|
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
NANOG is a homeobox transcription factor that functions as a core regulator of pluripotency and self-renewal in embryonic stem cells. Acting in concert with OCT4 (POU5F1) and SOX2, NANOG helps establish and maintain the transcriptional network that defines stem cell identity. Its expression is positively regulated by LIF/STAT3, FGF, and TGFβ/Activin signaling and negatively regulated by BMP signaling. NANOG is also highly expressed in cancer stem cells, where it can act as an oncogene that promotes tumor initiation, self-renewal, and resistance. As a central node in developmental biology and reprogramming research, NANOG transcriptional activity is widely monitored to track pluripotent and stem-like states.
Product Description & Applications
The NANOG Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of NANOG transcriptional activity in human or mouse cells. The reporter contains tandem repeats of consensus NANOG DNA-binding elements derived from the Tcf3 promoter, driving expression of a fluorescent (GFP, RFP, BFP, mCherry, EGFP, d2GFP) or luminescent (firefly, Renilla, or Gaussia luciferase) reporter. It is used to monitor pluripotency, self-renewal, reprogramming efficiency, and cancer stem cell activity. A range of antibiotic selection markers (blasticidin, hygromycin, puromycin, zeocin) supports establishment of stable reporter cell lines. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the NANOG transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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