NFκB-AP1 Reporter Lentivirus

Background

NF-κB and AP-1 are two of the most important inducible transcription factor families in mammalian cells. NF-κB, composed of Rel-family subunits, drives genes involved in inflammation, immune responses, cell survival, and proliferation. AP-1, formed by dimers of Jun, Fos, and related proteins, regulates genes controlling proliferation, differentiation, stress responses, and apoptosis. The two pathways are activated by overlapping stimuli, including cytokines, growth factors, and stress signals, and frequently cooperate to coordinate transcriptional programs in inflammation, embryonic development, lymphoid differentiation, and oncogenesis. Because both factors are central nodes of cellular signaling, monitoring their activity is valuable for studying inflammatory and immune responses and signal transduction.

Product Description & Applications

The NF-κB-AP1 Reporter Lentivirus is a transcription factor reporter system that detects activation of NF-κB and AP-1 in mammalian cells. The construct is built with tandem repeats of four NF-κB DNA-binding elements followed by four AP-1 DNA-binding elements, coupled to a minimal promoter and an optimized upstream enhancer that maximizes signal-to-noise, providing a fluorescent or luminescent readout of pathway activation under low-dose stimulation. A constitutive drug selection marker (Puromycin or Blasticidin) enables generation of stable polyclonal reporter cell lines suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, the product is well suited to studying NF-κB and AP-1 activity in primary and difficult-to-transfect cells for immunology and cell signaling research.

About This Product

This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the NFκB and AP1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.

Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.