| Field | Specification |
|---|---|
| Product Type | |
| Reporter | |
| Selection Marker | Hygromycin, Zeocin, Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
NR2F2, also known as COUP-TFII, is an orphan nuclear receptor and the most transcriptionally active member of the NR2F family, which also includes NR2F1 (COUP-TFI) and NR2F6 (EAR2). NR2F2 binds the AGGTCA DNA motif and can be fully activated by ligands such as 1-deoxysphingosines. It has conserved roles in neurogenesis and is required for cardiac development in mice and humans. NR2F2 also promotes the malignant state of squamous cell carcinomas by controlling tumor stemness and contributes to insulin-mediated breast cancer cell invasion and migration, making it relevant to developmental and cancer research.
Product Description & Applications
The NR2F2 Reporter Lentivirus is a transcription factor reporter system built with twelve direct repeats (DR0) of the NR2F AGGTCA DNA-binding motif upstream of a minimal promoter and a reporter gene, giving a sensitive fluorescent or luminescent readout of NR2F1/NR2F2 transcriptional activity. A wide range of reporters is available, including EGFP, mCherry, BFP2, d2GFP, GFP, RFP, firefly luciferase, Gaussia luciferase, and Renilla luciferase, with selection markers including blasticidin, hygromycin, puromycin, and zeocin. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.
It supports stable reporter cell line generation for studying NR2F2 activity in development and cancer.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP + Firefly Luc, mCherry, Renilla Luc, RFP + Firefly Luc, BFP2, Gaussia Luc, GFP, RFP reporter gene under the control of tandem consensus response elements specific for the NR2F2 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Hygromycin, Zeocin, Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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