| Field | Specification |
|---|---|
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
PAX2 is a member of the paired box (PAX) family of transcription factors, which contain a conserved paired-domain DNA-binding motif and act as master regulators of organ development. PAX2 is essential for the development of the kidney and urinary tract, the eye, the ear, and the central nervous system, where it governs cell proliferation, lineage specification, and tissue patterning. It also suppresses apoptosis in developing tissues. PAX2 expression is normally restricted after development, and its reactivation or mutation is associated with renal coloboma syndrome and contributes to several cancers, including renal and ovarian tumors, making it a target of interest in developmental biology and oncology.
Product Description & Applications
The PAX2 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of PAX2 transcriptional activity in mammalian cells. The construct contains tandem repeats of consensus PAX2 DNA-binding elements upstream of a reporter gene, so signal intensity reflects endogenous PAX2 activity. Stable lentiviral integration enables generation of polyclonal reporter cell lines with readout by fluorescence microscopy, flow cytometry, or luminometry. The system is applied to study PAX2 function in kidney and urinary tract development, tissue patterning, and developmental biology, and to investigate PAX2 dysregulation in disease. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the PAX2 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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