PPARδ Reporter Lentivirus

Background

PPARδ (PPARβ/δ), encoded by PPARD and also known as NUC1, is a ligand-activated nuclear receptor. Like other peroxisome proliferator-activated receptors, it heterodimerizes with the retinoid X receptor and binds PPAR response elements to activate or repress target genes. In the absence of ligand, PPARδ-RXR complexes associate with co-repressors and become transcriptionally active upon binding activating ligands such as fatty acids. PPARδ is especially important for fatty acid uptake, transport, and beta-oxidation, as well as insulin secretion and sensitivity. Cancer cells can upregulate PPARδ to withstand nutritional and energy stress, supporting survival and progression, making PPARδ relevant to both metabolic and cancer research.

Product Description & Applications

The PPARδ Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of PPARδ transcriptional activity in human or mouse cells. The construct contains tandem repeats of consensus PPARδ DNA-binding elements that are responsive to PPARδ, driving expression of a fluorescent (GFP, RFP, EGFP, d2GFP, mCherry) or luminescent (firefly luciferase) reporter. It is used to study PPARδ-mediated transcription, characterize ligands, and screen agonists and antagonists in the context of lipid metabolism and energy homeostasis. Antibiotic selection markers (puromycin or blasticidin) support establishment of stable reporter cell lines. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.

About This Product

This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, mCherry, GFP, RFP reporter gene under the control of tandem consensus response elements specific for the PPARδ transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.

Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.