PRE Reporter Lentivirus

Background

Progesterone is a steroid hormone central to the female reproductive cycle, pregnancy, and mammary gland development. Its physiological effects are mediated primarily by the progesterone receptor (PR), a ligand-activated nuclear receptor that, upon hormone binding, dimerizes and binds progesterone response elements (PRE) in target gene promoters to regulate transcription. The receptor exists as two main isoforms: PR-B acts as a strong transcriptional activator, while PR-A often functions as a modulator. PR-B activity is further stimulated through the MAPK-ERK pathway in the presence of progesterone. Progesterone signaling is important in endocrinology and reproductive biology, and dysregulated PR activity is relevant to breast and endometrial cancers.

Product Description & Applications

The PRE Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of progesterone signaling pathway activity in mammalian cells. The construct contains tandem repeats of the consensus progesterone receptor DNA-binding element, so reporter expression reports ligand-activated PR transcriptional output. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is used to study progesterone receptor signaling, hormone responsiveness, and PR-targeting compounds in endocrinology and cell signaling research. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.

About This Product

This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the Progesterone Signaling Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.

Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.