| Field | Specification |
|---|---|
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
SOX2 and OCT4 (POU5F1) are core transcription factors of the pluripotency network that maintains embryonic stem cell identity. They frequently act together, binding adjacent or composite DNA elements to cooperatively regulate target genes, including the pluripotency factor NANOG. The proximal NANOG promoter contains a highly conserved composite SOX2-OCT4 binding element that is essential for correctly regulated NANOG expression in embryonic stem cells. By controlling such targets, the SOX2-OCT4 partnership sustains self-renewal and pluripotency and is central to cellular reprogramming. Monitoring combined SOX2-OCT4 activity provides a direct readout of the pluripotent state in stem cell and developmental biology research.
Product Description & Applications
The SOX2-OCT4 Reporter Lentivirus is a composite transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of combined SOX2 and OCT4 activity in human or mouse cells. The reporter is built from a stem cell enhancer minigene containing six tandem repeats of the conserved SOX2-OCT4 composite element from the NANOG promoter, with native flanking sequence, and has been validated to read out SOX2 transcriptional activity in HEK293FT cells. Available reporters include GFP, RFP, BFP, mCherry, EGFP, d2GFP, and firefly, Renilla, or Gaussia luciferase, with antibiotic selection markers (blasticidin, hygromycin, puromycin, zeocin) for establishing stable reporter cell lines. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP + Firefly Luc, mCherry, Renilla Luc, RFP + Firefly Luc, BFP2, Gaussia Luc, GFP, RFP reporter gene under the control of tandem consensus response elements specific for the SOX2 and OCT4 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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