| Field | Specification |
|---|---|
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
STAT6 (signal transducer and activator of transcription 6) is a latent cytoplasmic transcription factor activated by the cytokines interleukin-4 and interleukin-13. Upon cytokine receptor engagement, JAK kinases phosphorylate STAT6, which dimerizes, translocates to the nucleus, and induces target genes. STAT6 is the central mediator of type 2 immune responses, driving Th2 differentiation, B cell immunoglobulin class switching to IgE, and the alternative activation of macrophages. Through these functions it is critically involved in allergy, asthma, and antiparasite immunity, and has emerging roles in tumor immunity. STAT6-dependent transcription provides a direct readout of IL-4/IL-13 and JAK/STAT signaling activity.
Product Description & Applications
The STAT6 Reporter Lentivirus is a transcription-factor reporter system that provides a sensitive fluorescent or luminescent readout of human or mouse STAT6 activity. The construct places a reporter gene under the control of tandem STAT6 response elements coupled to a minimal promoter, while a constitutively expressed selection marker supports stable cell line generation. Reporter options include fluorescent proteins and luciferase, with readout by fluorescence microscopy, flow cytometry, or luminometry.
The system is suited to studying STAT6 activity, JAK/STAT signaling, and type 2 immune responses, as well as screening pathway modulators. Particles are purified by PEG precipitation and sucrose gradient centrifugation, enabling effective transduction of difficult-to-transfect primary and thawed cells.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, Luc, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the STAT6 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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