Custom Lentiviral Particle Services

High-quality, custom lentiviral particle services on demand. Options include lentivirus packaged with custom shRNA, miRNA, sgRNA, or ORF cDNA constructs, or with custom reporter constructs for transcription factor response elements or gene promoters.

Ultra-purified 10-15 Day Turnaround 70% Knockdown Guaranteed In Vitro & In Vivo
10-15 Business Days Turnaround
≥70% Knockdown Efficiency Guaranteed
Ultra PEG & Sucrose-gradient Purified

End-to-End Lentiviral Vector Solutions

Comprehensive lentiviral vector services spanning construct design, high-titer packaging, and rigorous quality control. From plasmid construction to purified virus delivery, we support shRNA knockdown, ORF expression, CRISPR/Cas9 knockout, and reporter systems with scalable production suitable for in vitro and in vivo applications.

Custom shRNA Design miRNA Expression ORF cDNA Cloning LentiCRISPR sgRNA TF Reporter Systems Promoter Reporters Ultra-Purification 70% Knockdown Validated
Need consultation? Our lentiviral specialists can help select vectors and design constructs. info@lipexogen.com | 1-800-LIP-EXO
Vector design & cloning
Packaging & production
Ultra-purification
QC & Characterization

Advantages

Advantages of LipExoGen Custom Lentivirus

Validated shRNAs

We confirm the shRNA can provide at least 70% knockdown of your gene of interest using our superior fluorescence-based assay.

Ultra-Purified

All custom lentiviral particles are purified by PEG precipitation and sucrose-gradient centrifugation for highest quality.

Wide Vector Variety

Wide variety of lentiviral vectors to choose from with customizable promoters and reporters for your specific application.

In Vivo Detection

Detect transduced cells in vivo with luciferase or easily study gene regulation with bright fluorescent reporters (GFP, RFP).

Fast Turnaround

Quick delivery timeline—usually 10-15 business days from order confirmation to shipment.

Best Value

Take advantage of our optimized vectors and high-quality service for similar or reduced prices vs our competitors.

Exosome Engineering Available: We can also provide lentiviral particles engineered to recruit shRNA or miRNA to exosomes upon request.

Lentiviral Services

Lentiviral Services

Choose from our comprehensive portfolio of custom lentiviral particle services. All particles are ultra-purified and suitable for in vitro or in vivo applications.

Validated shRNA Lentiviral Particles

$595

High-titer custom lentiviral particles producing shRNAs for knockdown of your gene of interest. We guarantee the clone you receive will have at least 70% knockdown efficiency because we validate using our superior fluorescence-based reporter assay.

  • Knockdown validation data included
  • Fluorescence microscopy assay
  • Stable cell line establishment
  • Exosome recruitment available

Note: At least one construct in the mixture guaranteed to provide 70% or more knockdown efficiency. Simply provide your gene accession number.

Custom miRNA Lentivirus

$595

High-titer custom lentiviral particles for expression of the stem-loop or mature miRNA of your choice. We guarantee the clone you receive is functional for the expression of the mature miRNA you specified.

  • Stem-loop or mature miRNA
  • Functional expression guaranteed
  • Stable cell line generation
  • Exosome loading options

Custom cDNA ORF Lentivirus

$595

High-titer custom lentiviral particles for expression of your specified ORF cDNA fragment. We have created lentiviral vectors that express large size ORFs (3-6 kb) and generate much higher viral titers than currently used lentiviral vectors.

  • Large ORF capability (3-6 kb)
  • Higher viral titers than standard
  • Functional expression guaranteed
  • Accepts customer plasmids

Custom LentiCRISPR sgRNA Lentivirus

$695

Fast and easy CRISPR/Cas9 knockout service. We make two sgRNA sequences flanking the target site and clone them each to our all-in-one Cas9 sgRNA vector. Packaged as a mixture of two vectors to enhance knockout efficiency.

  • Dual sgRNA strategy
  • All-in-one Cas9 vector
  • ~1×10⁹ particles/500μl
  • Enhanced knockout efficiency

Custom TF Reporter Lentivirus

$595

Take your gene regulation research to a new level with custom high-titer lentiviral particles for mammalian expression of a fluorescent/bioluminescent reporter system responsive to your transcription factor of interest.

  • TF response elements included
  • GFP/RFP/Luciferase options
  • Functional reporter guarantee
  • Reporter activity images included

Custom Gene Promoter Lentivirus

$595*

Custom lentiviral particles for mammalian expression of a fluorescent/bioluminescent reporter system driven by the human or mouse proximal promoter of your choice. Add enhancer/repressor sequences if desired.

  • Human/Mouse proximal promoters
  • Enhancer/repressor addition
  • Custom regulatory elements
  • Fluorescent/bioluminescent readouts

*Note: Starting price for cloned DNA fragments <1.0 kb without difficult secondary structure.

Service Workflow

Service Workflow

Our streamlined process delivers high-quality lentiviral particles in 10-15 business days from order confirmation.

1

Submit Request

Provide gene accession number or sequence and select vector options

2

Design & Clone

Construct design, synthesis, and sequence verification

3

Packaging

Viral particle production and harvesting

4

Purification

PEG precipitation and sucrose-gradient centrifugation

5

QC & Delivery

Titer determination, validation, and shipment on dry ice

Turnaround Time
10-15 business days
Shipping
On dry ice, overnight delivery
Quality Guarantee
≥70% knockdown efficiency

Resources

Resources

Download service guides, checklists, and documentation to help plan your lentiviral project.

PDF

Service Guide

Complete guide to our lentiviral particle services, including vector maps, packaging specifications, and QC standards.

PDF

Inquiry Checklist

What to include in your request: gene accession number, promoter choice, payload details, desired volume, and use case.

Online

Vector Lists

Browse our premade vectors for fastest turnaround or general vectors that can be customized for your specific research needs.

PDF

Protocols & Safety

Handling guidelines, biosafety recommendations (BSL-2), storage instructions, and transduction protocols.

Need help selecting the right service?

Our scientists can help design your lentiviral constructs and select optimal vectors.

Quality Control Details

Quality Control Details

Comprehensive QC panel to validate purity, potency, and genome integrity before delivery. All custom lentiviral particles undergo rigorous testing.

Genome Titration

Standard

Quantitative PCR using primers targeting WPRE or specific transgene sequences to determine functional titer (TU/ml).

Method: qPCR / dPCR Time: 1-2 days

Knockdown Validation

Popular

Superior fluorescence-based reporter assay to confirm ≥70% knockdown efficiency of shRNA constructs. More reliable than standard qPCR.

Method: Fluorescence Microscopy Time: 3-5 days

Purity Analysis

Standard

SDS-PAGE with silver staining to visualize viral capsid proteins (p24 for HIV-based vectors) and assess contaminant levels.

Method: SDS-PAGE Time: 1-2 days

Replication Competent Lentivirus

Safety

PERT assay (Product Enhanced Reverse Transcriptase) and indicator cell line testing to detect replication-competent lentivirus (RCL).

Method: PERT / Indicator Cells Time: 2-3 weeks

Sterility & Mycoplasma

Standard

Endotoxin detection (LAL chromogenic assay), mycoplasma PCR, and bioburden plating for bacterial/fungal contamination.

Method: LAL / PCR / Plating Time: 3-5 days

Functional Expression

Optional

Western blot or functional assay to confirm expression of cDNA ORF or reporter gene (GFP/RFP/Luciferase) in target cells.

Method: Western / Imaging Time: 3-5 days

Included with Every Order

Genome Titer (qPCR)
Purity Assessment
Endotoxin Testing
Sequence Verification

FAQ

Do you support self-complementary AAV (scAAV)?
Yes — payload size constraints apply. We’ll confirm during scoping.
Can I specify a preferred supplier or platform?
Yes — list it in your request; we consider preferences during matching.
What is included in QC?
Titer (ddPCR or qPCR), VP:VG ratio, purity (SDS‑PAGE), endotoxin, mycoplasma; expanded options on request.

Ready to start an project?

Share your application, any details of your project, or special requirements. Our team will contact you after receiving your request.