| Field | Specification |
|---|---|
| Detection Method | |
| Form | Kit |
| Product Type | |
| Storage |
BlasTaq™ Probe One-Step RT-qPCR combines reverse transcription and TaqMan™ probe-based real-time qPCR into a single-tube reaction for maximum convenience and efficiency. This system includes the RT-qPCR Enzyme Mix and BlasTaq™ Probe 2X qPCR MasterMix, providing highly sensitive and specific performance for both cDNA synthesis and real-time amplification.
The proprietary RT-qPCR Enzyme Mix contains our next-generation BlasTaq™ DNA polymerase, along with specialized stabilizers and enhancers that optimize the two reactions in a seamless, single-step workflow. This design provides flexibility with a wide range of RNA templates and primer options, delivering a reliable, easy-to-use alternative to traditional two-step RT-qPCR.
The MasterMix is suitable for a variety of applications, including SNP genotyping, gene expression analysis, microarray validation, and high-throughput screening. ROX reference dye is supplied separately, ensuring universal compatibility with most qPCR instruments. For instrument-specific guidelines, please refer to the ROX Machine Compatibility Chart.
Product Features:
- Simple, one-step RT-qPCR reaction
- Flexibility with RNA templates and primer selection
- ROX reference dye provided separately
- Universally compatible with most qPCR instruments
- Included in abm's PCR Buffet Program
| Product Component | Quantity |
|---|---|
| BlasTaq™ Probe 2X qPCR MasterMix |
1.25 ml
|
| RT-qPCR Enzyme Mix |
100 rxn (40 μl)
|
| ROX Reference Dye | 15 μl |
| Nuclease-Free H2O |
1.0 ml
|
 |
ISO 13485:2016 MDSAP Certified |
| Component | Quantity |
|---|---|
| BlasTaq™ Probe 2X qPCR MasterMix | 1.25 ml |
| RT-qPCR Enzyme Mix | 100 rxn (40 μl) |
| ROX Reference Dye | 15 μl |
| Nuclease-Free H2O | 1.0 ml |
| Specification | Value |
|---|---|
| Enzyme Type | RT-qPCR Kit |
| Format | Kit |
| Storage Conditions | Store at -20°C. |
No, BlasTaq™ Probe 2X qPCR MasterMix does not contain an antibody. It includes dNTPs, BlasTaq™ DNA polymerase, MgCl₂, and proprietary buffer components, making it ideal for TaqMan probe-based real-time qPCR analysis and various applications like SNP genotyping and gene expression analysis.
BlasTaq™ DNA Polymerase is a next-generation, engineered Taq polymerase designed to offer faster extension rates, higher yields, and improved processivity compared to traditional wild-type Taq polymerase. It also provides enhanced sensitivity, and can reduce reaction times by up to 70% through a specialized reaction protocol.
This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
abm's qPCR MasterMixes don't contain ROX dye, but it is provided in a separate tube when you purchase any abm qPCR MasterMix. Some qPCR machines may require ROX, so please consult our machine compatibility list on the product page to see if your instrument needs it.
The recommended volume of ROX reference dye varies based on the qPCR instrument: No ROX equipment: No dye needed. Low ROX equipment: 1 μl/1.25 ml or 22.5 μl/25 ml. High ROX equipment: 11.5 μl/1.25 ml or 225 μl/25 ml.
Yes, this MasterMix is universally compatible with most qPCR instruments, as the ROX dye is provided separately for flexibility.
Probe-based assays, like those using TaqMan probes, rely on a specific probe that binds to the target DNA sequence and emits a fluorescent signal when cleaved during amplification. This provides high specificity for detecting the target sequence. Dye-based assays, like those using SYBR Green or EvaGreen, rely on a dye that binds to double-stranded DNA, emitting fluorescence as the amount of DNA increases during amplification. Dye-based assays are simpler but may be less specific since the dye binds to any double-stranded DNA, not just the target sequence. BlasTaq™ Probe 2X qPCR MasterMix is specifically designed for probe-based assays, offering higher specificity, while dye-based assays use MasterMixes like BlasTaq™ 2X qPCR MasterMix (Cat. No. G891).
Yes, BlasTaq™ Probe 2X qPCR MasterMix is suitable for high-throughput screening applications, as well as other applications like SNP genotyping and gene expression analysis.
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