| Field | Specification |
|---|---|
| Form | Kit |
| Product Type | |
| Storage |
MegaFi™ Pro One-Step RT-PCR is a highly efficient, all-in-one solution for performing both reverse transcription and PCR amplification in a single reaction. The kit includes two essential components: the RT-PCR enzyme mix, which combines OneScript® Hot Reverse Transcriptase (Cat. No. G593), MegaFi™ Pro Fidelity DNA Polymerase (Cat. No. G886) , and RNaseOFF Ribonuclease Inhibitor (Cat. No. G138), and the 2X One-Step RT-PCR Buffer, which contains gel loading dye and all other necessary reagents. The enzyme mix ensures exceptional sensitivity and high fidelity with over 2000X lower error rates, while the buffer is optimized with stabilizers and enhancers provides flexiblity in choosing desired primers to support robust reactions across a variety of RNA templates. This streamlined system simplifies the process, offering a reliable, one-step alternative to traditional two-step RT-PCR protocols.
Product Features:
- Enjoy simple, one-step RT-PCR reactions
- Extremely thermostable cDNA synthesis at 50-80°C
- Excellent protection against common ribonucleases
- Over 2000X lower error rates
- Included in abm's PCR Buffet Program
| Product Component | Quantity |
|---|---|
| RT-PCR Enzyme Mix | 100 rxn (400 µl) |
| 2X One-Step RT-PCR Buffer | 2 x 1.25 ml |
 |
ISO 13485:2016 MDSAP Certified |
| Component | Quantity |
|---|---|
| RT-PCR Enzyme Mix | 100 rxn (400 µl) |
| 2X One-Step RT-PCR Buffer | 2 x 1.25 ml |
| Specification | Value |
|---|---|
| Enzyme Type | RT-PCR Kit |
| Format | Kit |
| Storage Conditions | Store at -20°C. |
Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
We recommend using 1 ng to 2 μg of RNA per reaction.
This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
This product is abm’s next generation of One-Step RT-PCR Kits and is functionally equivalent to Cat. No. G174 and G174-dye (One-Step RT-PCR Kits), with improved performance. Contact our customer service team [contact ABM technical support] for more information.
The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
Although the kit components are supplied in two tubes (Enzyme Mix and 2X One-Step Buffer), the entire reverse transcription and PCR occur in a single reaction tube. This simplifies setup, saves time, and reduces contamination risk compared to two-step RT-PCR.
No. This kit is designed for end-point RT-PCR. For RT-qPCR, abm offers BlasTaq™ Probe One-Step RT-qPCR (Cat. No. G596).
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