| Field | Specification |
|---|---|
| Target | |
| Alternative names | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxysteroid 3-dehydrogenase; Delta-5-3-ketosteroid isomerase; Dihydrotestosterone oxidoreductase; Steroid Delta-isomerase; Trophoblast antigen FDO161G; HSD3B1; 3BH; HSDB3A |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HSD3B1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26H21 is a rabbit monoclonal antibody that recognizes 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (gene HSD3B1) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 42.3 kDa, and the supplier reports an observed band at about 42 kDa.
3β-hydroxysteroid dehydrogenase type 1 (HSD3B1) oxidizes and isomerizes Δ5-3β-hydroxysteroids to Δ4-3-ketosteroids, a step required to make progesterone, androgens, estrogens and corticosteroids. The type 1 isoform is expressed mainly in placenta and peripheral tissues such as skin and breast, and it is studied in steroid biosynthesis.
| Target | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (gene HSD3B1; UniProt P14060, human) |
|---|---|
| Clone | 26H21 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.3 kDa |
| Observed MW | 42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human placenta.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human HSD3B1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 in the endoplasmic reticulum membrane and mitochondrion membrane. Tissue expression noted by UniProt (human): Placenta and skin. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
H.-J. Wang et al. (2014) Resveratrol appears to protect against oxidative stress and steroidogenesis collapse in mice fed high-calorie and high-cholesterol diet. Andrologia. 10.1111/and.12231
