| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon regulatory factor 9; IRF-9; IFN-alpha-responsive transcription factor subunit; ISGF3 p48 subunit; Interferon-stimulated gene factor 3 gamma; ISGF-3 gamma; Transcriptional regulator ISGF3 subunit gamma; IRF9; ISGF3G |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IRF-9 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21I34 is a rabbit monoclonal antibody that recognizes interferon regulatory factor 9 (gene IRF9) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 43.7 kDa, and the supplier reports an observed band at about 48 kDa.
Interferon regulatory factor 9 (IRF9) is the DNA-binding partner of STAT1 and STAT2 in the ISGF3 complex. After type I interferon receptor signaling through JAK1 and TYK2, ISGF3 enters the nucleus and binds ISRE elements to switch on interferon-stimulated genes, making IRF9 essential for antiviral defense.
| Target | Interferon regulatory factor 9 (gene IRF9; UniProt Q00978, human) |
|---|---|
| Clone | 21I34 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.7 kDa |
| Observed MW | 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human IRF-9.
Reactivity Notes
The supplier lists reactivity with human. UniProt places interferon regulatory factor 9 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
