| Field | Specification |
|---|---|
| Target | |
| Alternative names | NPC intracellular cholesterol transporter 1; Niemann-Pick C1 protein; NPC1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Niemann Pick C1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18N07 is a rabbit monoclonal antibody that recognizes NPC intracellular cholesterol transporter 1 (gene NPC1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 142.2 kDa, and the supplier reports an observed band at about 180 kDa.
NPC1 is a large lysosomal membrane protein that exports cholesterol from late endosomes and lysosomes. It receives LDL-derived cholesterol from the soluble protein NPC2 and moves it across the lysosomal membrane. Loss of NPC1 causes Niemann–Pick disease type C, and NPC1 also serves as the intracellular receptor for Ebola virus entry.
| Target | NPC intracellular cholesterol transporter 1 (gene NPC1; UniProt O15118, human) |
|---|---|
| Clone | 18N07 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 142.2 kDa |
| Observed MW | 180 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human niemann Pick C1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places NPC intracellular cholesterol transporter 1 in the late endosome membrane and lysosome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xuebing Cheng et al. (2020) Bioinformatic and biochemical findings disclosed anti-hepatic steatosis mechanism of calycosin. Bioorganic Chemistry. 10.1016/j.bioorg.2020.103914
