| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitogen-activated protein kinase 14;MAP kinase 14;MAPK 14;2.7.11.24 ;Cytokine suppressive anti-inflammatory drug-binding protein;CSAID-binding protein;CSBP;MAP kinase MXI2;MAX-interacting protein 2;Mitogen-activated protein kinase p38 alpha;MAP kinase p38 alpha;Stress-activated protein kinase 2a;SAPK2a;MAPK14;CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human p38 MAPK |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EGC-13 is a rabbit monoclonal antibody that recognizes mitogen-activated protein kinase 14 (gene MAPK14) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 41.3 kDa; the supplier reports observed bands at 41, 38 kDa.
p38α (MAPK14) is the best-studied p38 MAP kinase and is expressed in most cells. Activated by MKK3 and MKK6 in response to inflammatory cytokines, LPS and cellular stress, it phosphorylates kinases such as MK2 and many transcription factors to control cytokine production, differentiation and cell death, making it a common target in inflammation research.
| Target | Mitogen-activated protein kinase 14 (gene MAPK14; UniProt Q16539, human) |
|---|---|
| Clone | EGC-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.3 kDa |
| Observed MW | 41, 38 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human Jurkat cells, human SW620 cells, human Caco-2 cells, human HeLa cells, rat heart tissue, rat lung tissue, mouse heart tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Samples with confirmed staining (IHC): human brain tissue, human thyroid cancer tissue, human prostate cancer tissue, human astrocytoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide based on the sequence of human p38 MAPK.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitogen-activated protein kinase 14 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Brain, heart, placenta, pancreas and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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