| Field | Specification |
|---|---|
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Rat Primary Bone Marrow Monocytes are rat frozen primary cells from bone marrow used for innate immune signaling, phagocytic response, and inflammatory pathway studies. These cells display suspension, round growth characteristics.
Key elements and design rationale
- Biological source: Rat (R. norvegicus)
- Tissue origin: Bone Marrow
- Growth properties: Suspension, round
- Format: Frozen
- Biosafety level: II
- Culture context: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) . Leukocyte Medium Kit (TM164) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
Monocyte- and macrophage-lineage cells coordinate innate immune sensing, cytokine production, phagocytosis, and tissue-specific inflammatory responses.
Research relevance and current trends
- Primary innate immune cell models are widely used to assess activation-state plasticity and cytokine output.
- Researchers often study tissue-specific macrophage phenotypes or donor-dependent inflammatory responses.
- Co-culture systems help reveal crosstalk with stromal, epithelial, endothelial, or parenchymal cells.
Common research applications
- Measure inflammatory mediator release and activation markers after stimulation.
- Use in phagocytosis-focused or co-culture assays requiring primary innate immune cells.
- Evaluate tissue-specific immune crosstalk in organ-relevant model systems.
Product-specific data supplied for this listing
- Growth Conditions: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) . Leukocyte Medium Kit (TM164) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
- Disclaimer: 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 1 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 8-10ml of pre-warmed, complete growth media. Centrifuge cells at 200xg for 5 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask or proceed with designed experiments immediately.
- Incubate the cells at the recommended conditions. These cells will not proliferate in culture and cannot be passaged.
- Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
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