ABCG2 Antibody

Overview

This polyclonal anti-ABCG2 antibody raised in Rabbit, tested in ELISA, IF. Designed to detect ABCG2 in Human for tags & cell markers research.

Key elements and design rationale

• Immunogen: Recombinant Human ATP-binding cassette sub-family G member 2 protein (296-371AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.

Biological background

ABCG2 (also: ABC transporter, ABC15, ABCG 2, ABCG2, ABCG2_HUMAN, ABCP, ATP binding cassette sub family G (WHITE) member 2, ATP binding cassette transporter G2, ATP-binding cassette sub-family G member 2, BCRP, BCRP1, BMDP, Breast cancer resistance protein, CD338, CDw338, CDw338 antigen , EST157481, GOUT1, MGC102821, Mitoxantrone resistance associated protein, Mitoxantrone resistance-associated protein, MRX, Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2, MXR, MXR1, Placenta specific ATP binding cassette transporter, Placenta specific MDR protein, Placenta-specific ATP-binding cassette transporter, UAQTL1) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current ABCG2 annotation relevant to tags & cell markers.

Common research applications

• ELISA: Quantify soluble target; ensure samples within the linear detection range.
• IF: Subcellular localization in fixed cells/sections; optimize fixation.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Human; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (12556221)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.