| Field | Specification |
|---|---|
| Alternative Names | Adapter-related protein complex 1 subunit sigma-1C; Adaptor protein complex AP 1 sigma 1C subunit; Adaptor protein complex AP-1 subunit sigma-1C; Adaptor related protein complex 1 sigma 3 subunit; AP-1 complex subunit sigma-3; Ap1s3; AP1S3_HUMAN; Clathrin assembly protein complex 1 sigma-1C small chain; Golgi adaptor HA1/AP1 adaptin sigma-1C subunit; Sigma 1C subunit of AP-1 clathrin; Sigma-adaptin 1C; Sigma1C-adaptin |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human AP-1 complex subunit sigma-3 protein (1-104AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-AP1S3 antibody raised in Rabbit, conjugated with Biotin, with confirmed utility in ELISA, IHC. Designed to detect AP1S3 in Human, supporting researchers in others contexts.
Key elements and design rationale
- Immunogen: Recombinant Human AP-1 complex subunit sigma-3 protein (1-104AA) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.
- Host (Rabbit): Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.
- Polyclonal: Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.
- Isotype (IgG): Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A/G compatibility.
- Purification (Protein G purification): Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.
- Biotin conjugate: Signal amplification via streptavidin/avidin-coupled reporters. Block endogenous biotin in liver, kidney, or biotin-supplemented samples.
Biological background
AP1S3 (also known as Adapter-related protein complex 1 subunit sigma-1C, Adaptor protein complex AP 1 sigma 1C subunit, Adaptor protein complex AP-1 subunit sigma-1C, Adaptor related protein complex 1 sigma 3 subunit, AP-1 complex subunit sigma-3, Ap1s3, AP1S3_HUMAN, Clathrin assembly protein complex 1 sigma-1C small chain, Golgi adaptor HA1/AP1 adaptin sigma-1C subunit, Sigma 1C subunit of AP-1 clathrin, Sigma-adaptin 1C, Sigma1C-adaptin) is a protein studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of AP1S3 biology relevant to others.
Common research applications
- ELISA: Sandwich or indirect ELISA quantifies soluble/secreted target in biological fluids or cell culture supernatants.
- IHC: IHC visualizes target expression in FFPE or frozen sections; optimize antigen retrieval and secondary detection per tissue type.
Notes for experimental interpretation
- Isotype controls: Include IgG from Rabbit at equivalent concentration to assess non-specific background.
- Cross-reactivity/isoforms: Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.
- Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.
- Species reactivity: Confirmed for Human; extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
AP1S3 is a protein target in Human biology. This polyclonal antibody raised in Rabbit detects AP1S3 and is validated for ELISA, IHC applications.
This antibody is reported reactive against Human. Immunogen derived from Human. Cross-reactivity with unlisted species should not be assumed — contact us or consult the datasheet for homology data.
Supplier reports performance in ELISA, IHC. Dilution optimization, blocking, and appropriate controls are required per format. Contact BioHippo for recommended working concentrations.
Biotin enables streptavidin-amplified detection with high sensitivity. Block endogenous biotin (liver, kidney, biotin-supplemented media) to minimize background.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — lysate/cell line expressing AP1S3; (3) Negative control — siRNA KD or KO sample to confirm specificity; (4) Dilution linearity — verify proportional signal decrease for quantitative applications.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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