| Field | Specification |
|---|---|
| Alternative Names | Ctcfl; Boris; Transcriptional repressor CTCFL; Brother of the regulator of imprinted sites; CCCTC-binding factor; CTCF paralog; CTCF-like protein |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Mouse Transcriptional repressor CTCFL protein (1-636AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-Ctcfl antibody raised in Rabbit, with confirmed utility in ELISA, WB. Designed to detect Ctcfl in Mouse, Human, supporting researchers in epigenetics and nuclear signaling contexts.
Key elements and design rationale
- Immunogen: Recombinant Mouse Transcriptional repressor CTCFL protein (1-636AA) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.
- Host (Rabbit): Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.
- Polyclonal: Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.
- Isotype (IgG): Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A/G compatibility.
- Purification (Protein G purification): Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.
Biological background
Ctcfl (also known as Ctcfl, Boris, Transcriptional repressor CTCFL, Brother of the regulator of imprinted sites, CCCTC-binding factor, CTCF paralog, CTCF-like protein) is a protein studied in Mus musculus (Mouse) systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of Ctcfl biology relevant to epigenetics and nuclear signaling.
Common research applications
- ELISA: Sandwich or indirect ELISA quantifies soluble/secreted target in biological fluids or cell culture supernatants.
- WB: Western blot confirms target MW and detects isoforms/fragments under denaturing conditions.
Notes for experimental interpretation
- Isotype controls: Include IgG from Rabbit at equivalent concentration to assess non-specific background.
- Cross-reactivity/isoforms: Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.
- Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.
- Species reactivity: Confirmed for Mouse, Human; extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
Ctcfl is a protein target in Mouse, Human biology. This polyclonal antibody raised in Rabbit detects Ctcfl and is validated for ELISA, WB applications.
This antibody is reported reactive against Mouse, Human. Immunogen derived from Mus musculus (Mouse). Cross-reactivity with unlisted species should not be assumed — contact us or consult the datasheet for homology data.
Supplier reports performance in ELISA, WB. Dilution optimization, blocking, and appropriate controls are required per format. Contact BioHippo for recommended working concentrations.
This non-conjugated (IgG) antibody requires a secondary anti-rabbit IgG conjugated to HRP, AP, fluorophore, or biotin. Match the secondary to the host (Rabbit) and isotype (IgG).
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — lysate/cell line expressing Ctcfl; (3) Negative control — siRNA KD or KO sample to confirm specificity; (4) Dilution linearity — verify proportional signal decrease for quantitative applications.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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