| Field | Specification |
|---|---|
| Alternative Names | CCL 28; CCL28; Ectoderm neural cortex protein 1; Ectoderm-neural cortex protein 1; Ectodermal neural cortex (with BTB like domain); ENC 1; ENC-1; ENC1; ENC1_HUMAN; FLJ39259; Kelch like 35; Kelch like 37; Kelch-like protein 37; KLHL 35; KLHL35; KLHL37; NRPB; Nuclear matrix protein NRP/B; Nuclear restricted protein BTB domain like (brain); OTTHUMP00000128235; OTTHUMP00000222054; p53 induced gene 10 protein; p53-induced gene 10 protein; PIG 10; PIG10; TP53I10; Tumor protein p53 inducible protein 10 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Synthesized peptide derived from human ENC1 |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-ENC1 antibody raised in Rabbit, with confirmed utility in ELISA, WB. Designed to detect ENC1 in Human, Mouse, supporting researchers in tags & cell markers contexts.
Key elements and design rationale
- Immunogen: Synthesized peptide derived from human ENC1 — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.
- Host (Rabbit): Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.
- Polyclonal: Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.
- Isotype (IgG): Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A/G compatibility.
- Purification (Antigen affinity purification): Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.
Biological background
ENC1 (also known as CCL 28, CCL28, Ectoderm neural cortex protein 1, Ectoderm-neural cortex protein 1, Ectodermal neural cortex (with BTB like domain), ENC 1, ENC-1, ENC1, ENC1_HUMAN, FLJ39259, Kelch like 35, Kelch like 37, Kelch-like protein 37, KLHL 35, KLHL35, KLHL37, NRPB, Nuclear matrix protein NRP/B, Nuclear restricted protein BTB domain like (brain), OTTHUMP00000128235, OTTHUMP00000222054, p53 induced gene 10 protein, p53-induced gene 10 protein, PIG 10, PIG10, TP53I10, Tumor protein p53 inducible protein 10) is a protein studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of ENC1 biology relevant to tags & cell markers.
Common research applications
- ELISA: Sandwich or indirect ELISA quantifies soluble/secreted target in biological fluids or cell culture supernatants.
- WB: Western blot confirms target MW and detects isoforms/fragments under denaturing conditions.
Notes for experimental interpretation
- Isotype controls: Include IgG from Rabbit at equivalent concentration to assess non-specific background.
- Cross-reactivity/isoforms: Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.
- Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.
- Species reactivity: Confirmed for Human, Mouse; extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
ENC1 is a protein target in Human, Mouse biology. This polyclonal antibody raised in Rabbit detects ENC1 and is validated for ELISA, WB applications.
This antibody is reported reactive against Human, Mouse. Immunogen derived from Human. Cross-reactivity with unlisted species should not be assumed — contact us or consult the datasheet for homology data.
Supplier reports performance in ELISA, WB. Dilution optimization, blocking, and appropriate controls are required per format. Contact BioHippo for recommended working concentrations.
This non-conjugated (IgG) antibody requires a secondary anti-rabbit IgG conjugated to HRP, AP, fluorophore, or biotin. Match the secondary to the host (Rabbit) and isotype (IgG).
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — lysate/cell line expressing ENC1; (3) Negative control — siRNA KD or KO sample to confirm specificity; (4) Dilution linearity — verify proportional signal decrease for quantitative applications.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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