| Field | Specification |
|---|---|
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Enhanced Primary Human Liver Sinusoidal Endothelial Cells are expanded primary cells that retain the properties of primary liver sinusoidal endothelial cells allowing for a reliable in vitro research model. These cells are ready-to-use for applications in research and development, such as viral infections, screening, co-cultures, transient transfection, and for toxicity studies. The product is supplied as frozen cells.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Liver
- Growth properties: Adherent, endothelial-like
- Format: Frozen
- Reported expression/markers: MMR+, CD31+
- Seeding guidance: 10,000 cells/cm²
- Biosafety level: II
Endothelial cells line the luminal surface of blood vessels and regulate barrier properties, hemostatic balance, immune cell trafficking, angiogenic signaling, and tissue-specific exchange processes.
Research relevance and current trends
- Primary endothelial models are used to study barrier integrity, inflammatory activation, and permeability changes in tissue-specific vascular beds.
- Co-culture systems with immune, stromal, or parenchymal cells are increasingly used to model microenvironment-dependent signaling.
- Researchers often compare donor, vessel-bed, or organ-specific phenotypes to capture biologically relevant heterogeneity.
Common research applications
- Assess barrier function, adhesion molecule regulation, or cytokine-driven endothelial activation in vitro.
- Model angiogenic responses, matrix interactions, and vessel-bed-specific signaling under defined culture conditions.
- Use in co-culture or transwell systems to study leukocyte transmigration and paracrine crosstalk.
Product-specific data supplied for this listing
- Growth Conditions: PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Human Liver Sinusoidal Endothelial Cell Thawing Medium (TM111) + Human Liver Sinusoidal Endothelial Cell Media Kit (TM112), 37.0°C, 5% CO₂.Note: TM111 is required for optimal recovery of cells post-thaw. Cells are grown using TM112.
- Seeding Density (cells/cm²): 10,000
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Marker panels should be interpreted together with morphology and functional readouts rather than as a standalone identity measure.
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. Do not shake vial.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 50ml sterile conical tube containing 20ml of pre-warmed, Human Liver Sinusoidal Endothelial Cell Thawing Medium (TM111). Centrifuge cells at 280×g for 5 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in 1 ml of pre-warmed, complete Human Liver Sinusoidal Endothelial Cell Growth Medium (TM1112) and seed at 10,000 cells/cm2 in the appropriate G422 coated culture vessel.
- Incubate the cells at the recommended conditions.
- After 24 hours, add fresh Human Liver Sinusoidal Endothelial Cell Growth Medium (TM112). Change media every other day with fresh Human Liver Sinusoidal Endothelial Cell Growth Medium (TM112) until cells become 70-80% confluent.
- Aspirate the culture media, wash the adherent monolayer with 1X PBS, then and add 2-3ml of pre-warmed TrypLE Express to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize TrypLE Express by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 280xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Seed cells at 10,000 cells/cm2 in the appropriate G422 coated culture vessel.
- Incubate the cells at the recommended conditions.
- Allow the cells to expand over the next 2-3 days then perform experimental assays as desired.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
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