| Field | Specification |
|---|---|
| Alternative Names | GAPDH; GAPDGlyceraldehyde-3-phosphate dehydrogenase; GAPDH; EC 1.2.1.12; Peptidyl-cysteine S-nitrosylase GAPDH; EC 2.6.99.- |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase protein (2-333AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-GAPDH antibody raised in Rabbit, conjugated with Biotin, with confirmed utility in ELISA. It is designed to detect GAPDH protein in Cricetulus griseus and supports researchers working in others contexts where consistent antibody performance is required.
Key elements and design rationale
- Immunogen: Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase protein (2-333AA) — the immunizing antigen determines the epitope region; confirm epitope compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents. Use matched secondaries to avoid no-signal or cross-reactivity issues.
- Polyclonal format: Recognizes multiple epitopes, providing robust signal across varied preparations and species variants. Inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Compatible with standard Protein A/G purification and widely supported by secondary reagents. Include an isotype-matched control at equivalent concentration.
- Purification (Protein G purification): Enriches for specific immunoglobulin classes, reducing non-specific populations and improving signal-to-noise.
- Biotin conjugate: Amplified detection via streptavidin/avidin reporters. Block endogenous biotin in biotin-rich tissues prior to staining.
Biological background
GAPDH (also referred to as GAPDH, GAPDGlyceraldehyde-3-phosphate dehydrogenase, GAPDH, EC 1.2.1.12, Peptidyl-cysteine S-nitrosylase GAPDH, EC 2.6.99.-) is a protein target studied in Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection and experimental design. Consult UniProt, NCBI Gene, and primary literature for current annotation of GAPDH biology, including known isoforms, interactors, and disease-relevant expression patterns in others.
Common research applications
- ELISA: Sandwich or indirect ELISA can quantify soluble target in biological fluids or culture supernatants. Ensure samples fall within the linear detection range.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins or isoforms. Orthogonal validation (siRNA, KO lysate, recombinant protein) is recommended to confirm signal specificity.
- Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) can affect performance. Pilot dilution linearity and spike-recovery experiments are recommended for quantitative studies.
- Species reactivity: Confirmed for Cricetulus griseus. Extrapolation to untested species requires empirical validation given potential epitope sequence divergence.
This product is a polyclonal antibody purified from rabbit antiserum.
GAPDH is a protein target in Cricetulus griseus biology. This polyclonal antibody raised in Rabbit is designed to detect GAPDH in ELISA applications, with IgG isotype.
This antibody is reported reactive against Cricetulus griseus. The immunogen was derived from Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus). Cross-reactivity with other species should not be assumed without documented data or empirical testing.
The supplier reports performance in ELISA. Each application requires independent dilution optimization, blocking conditions, and appropriate controls. Performance in unlisted applications requires empirical testing.
Biotin conjugation supports signal amplification via streptavidin/avidin-coupled reporters. Block endogenous biotin in biotin-rich tissues (liver, kidney) or biotin-supplemented culture media before staining.
Recommended: (1) Isotype control — IgG from Rabbit at matching concentration for non-specific background; (2) Positive control — known GAPDH-expressing cell/tissue; (3) Negative control — knockdown or knockout sample to confirm signal specificity; (4) Dilution linearity — verify proportional signal decrease to confirm quantitative linearity in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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