Human Primary CD3+ Blood Cells

Overview

Human Primary CD3+ Blood Cells are human frozen primary cells from blood used for T-cell activation, signaling, proliferation, and immune response studies. These cells display suspension, round growth characteristics.

Key elements and design rationale

• Biological source: Human (H. sapiens)
• Tissue origin: Blood
• Growth properties: Suspension, round
• Format: Frozen
• Reported expression/markers: CD3+
• Biosafety level: II
• Culture context: PriGrow X Series Medium (TM5401) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.

Primary T cells are central to adaptive immune responses and are commonly studied for activation-state transitions, cytokine signaling, proliferation, and cell–cell communication.

Research relevance and current trends

• Primary T-cell assays frequently evaluate activation markers, cytokine production, and proliferation under defined stimuli.
• Subset-specific or enriched T-cell preparations support mechanistic studies of signaling and donor heterogeneity.
• Co-culture and conditioned-media systems are often used to model cross-talk with antigen-presenting or stromal cells.

Common research applications

• Assess activation, proliferation, and cytokine responses after stimulation.
• Use in immune co-culture assays to study cell–cell signaling and response modulation.
• Profile donor- or condition-dependent changes in phenotype and functional readouts.

Product-specific data supplied for this listing

• Growth Conditions: PriGrow X Series Medium (TM5401) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
• Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
• Disclaimer: 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.

Notes for experimental interpretation

• Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
• Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
• Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
• Marker panels should be interpreted together with morphology and functional readouts rather than as a standalone identity measure.

SKU:BHC10900066

Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.