| Field | Specification |
|---|---|
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Human Primary Peripheral Blood Mononuclear Cells are human frozen primary cells from blood used for immune profiling, cytokine response, and ex vivo stimulation studies. These cells display suspension, round growth characteristics.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Blood
- Growth properties: Suspension, round
- Format: Frozen
- Donor history: Normal tissue
- Biosafety level: II
- Culture context: PriGrow X Series Medium (TM4103) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
Peripheral blood mononuclear cells comprise lymphoid and monocyte populations that are widely used for immune phenotyping, activation assays, cytokine analysis, and donor-response studies.
Research relevance and current trends
- Primary PBMC models are used to study donor-to-donor variability in cytokine response and activation-state changes.
- Multiparameter phenotyping and perturbation assays remain common for evaluating immune composition and function.
- Investigators increasingly pair PBMCs with disease-relevant soluble factors or co-culture systems to model immune crosstalk.
Common research applications
- Measure cytokine release, activation markers, or population shifts after stimulation.
- Use for phenotyping, functional immune assays, or donor-comparison studies.
- Incorporate into co-culture designs to evaluate immune interactions with other primary cell types.
Product-specific data supplied for this listing
- Growth Conditions: PriGrow X Series Medium (TM4103) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
- Disclaimer: 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10⁶ cells/ml.
- Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
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