NEDD9 Antibody

Overview

This polyclonal anti-NEDD9 antibody raised in Rabbit, tested in ELISA, IHC. Designed to detect NEDD9 in Human for cell biology research.

Key elements and design rationale

• Immunogen: Recombinant Human Enhancer of filamentation 1 protein (68-151AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.

Biological background

NEDD9 (also: Cas like docking, Cas scaffolding protein family member 2, CAS-L, CAS2, CasL, CASL_HUMAN, CASS2, Crk associated substrate related, Crk associated substrate related protein, CRK-associated substrate-related protein, dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)), dJ49G10.2, dJ761I2.1 (enhancer of filamentation (HEF1)), dJ761I2.1, Enhancer of filamentation 1, Enhancer of filamentation 1 p55, HEF 1, HEF1, NEDD-9, Nedd9, Neural precursor cell expressed developmentally down-regulated protein 9, P105, Renal carcinoma antigen NY-REN-12) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current NEDD9 annotation relevant to cell biology.

Common research applications

• ELISA: Quantify soluble target; ensure samples within the linear detection range.
• IHC: Tissue expression in FFPE/frozen sections; optimize antigen retrieval.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Human; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (12554366)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.