| Field | Specification |
|---|---|
| Alternative Names | PP 7; PP7; PPE1_HUMAN; PPEF 1; PPEF; PPEF-1; Ppef1; PPP 7C; PPP7C; PPP7CA; Protein phosphatase 7, catalytic subunit, alpha isozyme; Protein phosphatase EF hand calcium binding domain 1; Protein phosphatase serine/threonine type with EF hands; Protein phosphatase with EF calcium binding domain; Protein phosphatase with EF calcium-binding domain; Protein phosphatase with EF hand calcium binding domain 1; Serine/threonine protein phosphatase 7; Serine/threonine protein phosphatase with EF hands 1; Serine/threonine-protein phosphatase 7; Serine/threonine-protein phosphatase with EF-hands 1 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human Serine/threonine-protein phosphatase with EF-hands 1 protein (301-500AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-PPEF1 antibody raised in Rabbit, conjugated with Biotin, with confirmed utility in ELISA. Designed to detect PPEF1 in Human, supporting researchers in signal transduction contexts.
Key elements and design rationale
- Immunogen: Recombinant Human Serine/threonine-protein phosphatase with EF-hands 1 protein (301-500AA) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.
- Host (Rabbit): Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.
- Polyclonal: Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.
- Isotype (IgG): Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A/G compatibility.
- Purification (Antigen affinity purification): Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.
- Biotin conjugate: Signal amplification via streptavidin/avidin-coupled reporters. Block endogenous biotin in liver, kidney, or biotin-supplemented samples.
Biological background
PPEF1 (also known as PP 7, PP7, PPE1_HUMAN, PPEF 1, PPEF, PPEF-1, Ppef1, PPP 7C, PPP7C, PPP7CA, Protein phosphatase 7, catalytic subunit, alpha isozyme, Protein phosphatase EF hand calcium binding domain 1, Protein phosphatase serine/threonine type with EF hands, Protein phosphatase with EF calcium binding domain, Protein phosphatase with EF calcium-binding domain, Protein phosphatase with EF hand calcium binding domain 1, Serine/threonine protein phosphatase 7, Serine/threonine protein phosphatase with EF hands 1, Serine/threonine-protein phosphatase 7, Serine/threonine-protein phosphatase with EF-hands 1) is a protein studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of PPEF1 biology relevant to signal transduction.
Common research applications
- ELISA: Sandwich or indirect ELISA quantifies soluble/secreted target in biological fluids or cell culture supernatants.
Notes for experimental interpretation
- Isotype controls: Include IgG from Rabbit at equivalent concentration to assess non-specific background.
- Cross-reactivity/isoforms: Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.
- Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.
- Species reactivity: Confirmed for Human; extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
PPEF1 is a protein target in Human biology. This polyclonal antibody raised in Rabbit detects PPEF1 and is validated for ELISA applications.
This antibody is reported reactive against Human. Immunogen derived from Human. Cross-reactivity with unlisted species should not be assumed — contact us or consult the datasheet for homology data.
Supplier reports performance in ELISA. Dilution optimization, blocking, and appropriate controls are required per format. Contact BioHippo for recommended working concentrations.
Biotin enables streptavidin-amplified detection with high sensitivity. Block endogenous biotin (liver, kidney, biotin-supplemented media) to minimize background.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — lysate/cell line expressing PPEF1; (3) Negative control — siRNA KD or KO sample to confirm specificity; (4) Dilution linearity — verify proportional signal decrease for quantitative applications.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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