| Field | Specification |
|---|---|
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Primary Rat (Wistar Han) Hepatocytes, Plateable are rat frozen primary cells from liver used for drug metabolism, hepatotoxicity, liver function, and cell-based assay development. These cells display adherent, polygonal growth characteristics.
Key elements and design rationale
- Biological source: Rat (R. norvegicus)
- Tissue origin: Liver
- Growth properties: Adherent, polygonal
- Format: Frozen
- Donor history: Normal tissue
- Biosafety level: II
- Culture context: PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels.
Hepatocytes are central to xenobiotic metabolism, intermediary metabolism, protein synthesis, and bile-related functions. Primary hepatocyte models are widely used when physiologic metabolic capacity is important.
Research relevance and current trends
- Primary hepatocyte cultures remain important for metabolism, transporter, and toxicity studies requiring liver-relevant function.
- Longer-term maintenance and co-culture approaches are used to preserve phenotype for repeated-dose or chronic-exposure designs.
- Researchers often profile donor-to-donor variability in metabolism and inducible response pathways.
Common research applications
- Evaluate metabolism, transporter activity, or toxicity responses in liver-relevant in vitro systems.
- Measure inducible gene expression or secreted markers after compound or cytokine treatment.
- Use in co-culture workflows to model hepatic crosstalk with stromal or immune cell populations.
Product-specific data supplied for this listing
- Growth Conditions: PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0106) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: TM0106 contains both TM0106-A (Thawing and Plating Supplement for TM0106) as well as TM0106-B (Cell Maintenance Supplement for T0106) which can be added to the basal media to create the complete media desired.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
- Disclaimer: 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
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