| Field | Specification |
|---|---|
| Product Format | Frozen |
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Overview
This highly tumorigenic cell line is derived from a primary tumor generated by the s.c. injection of GhrasT-NIH/3T3 (Cat. T3317) cells into the hindquarters of an outbred NIH Swiss mouse. *Note: Identical injections with this T1-A cell line produced 25x more primary tumors (20 of 20 = 100% mice injected) than were produced with injections of its parental transformed GhrasT-NIH/3T3 cell line (4 of 104 = 3.8% mice injected).
Key elements and design rationale
- Model identity: Primary Tumor Cell Line (T1-A ) is supplied as a tumor cell line derived from Mouse muscle.
- Growth properties: Adherent, fibroblast
- Growth conditions: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) . PriGrow III (TM003) + 10% FBS(Regular*) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Avoid excessive alkalinity in media and renew twice weekly. *Do not heat-inactivate
- Product format: Frozen, BSL-2
This cell-based model is generally used in liver cancer biology, phenotype comparison, and assay development studies. Donor/background information is available for contextual interpretation.
Biological background
This cell line is part of an eight cell R/J Model of Metastatic Progression designed using the NIH Swiss mice species to acquire isogenic cell types (T4138, T8981,T3317, T8144-T8148) that capture genetic/phenotypic stages in the progression to highly metastatic growth within this same species. Compared to some other models, the metastasis related changes captured in this unique progression model are easier to discern above their less noisy isogenic background and have progressed within their native NIH Swiss in vivo micro-environments. This isogenic R/J Metastasis Cell Model was produced by an in vitro/in vitro six-step progression from mortal normal NIH Swiss mouse cells (Cat. T4138) that were used to produce immortal pre-cancer NIH/3T3 cells (Cat. T8981) that were transfected with the human HRAS oncogene to produce transformed cancer GhrasT-NIH/3T3 cells (Cat. T3317). Then beginning with a series of in vivo/in vitro progressive transfers of GhrasT-NIH/3T3 cells produced primary tumor T1-A cells (Cat. T8144) that produced local metastatic lesion T2-A cells (Cat. T8145) that simultaneously produced twin distant metastatic lung lesion T3-PA cells (Cat. T8146) with its twin distant metastatic liver lesion cells (Cat. T1847). The liver derived cells produced highly distant metastatic lung lesion cells (Cat T1848) which retained the human HRAS oncogene and they demonstrated a highly metastatic phenotype as they produced wide-spread simultaneous external and internal metastases in multiple sites in multiple mice when injected i.v. into nude NIH Swiss mice.Complete list of cells included in the R/J Model: Cat. T4138 - Normal Primary Diploid Mortal NIH Swiss Mouse Embryonic CellsCat. T8981 - NIH-3T3 CellsCat. T3317 - HRAS Stably Expressing NIH/3T3 Cell Line (GhrasT-NIH/3T3)Cat. T8144 - Primary Tumor Cell Line (T1-A ) Cat. T8145 - Local Metastasis Tumor Cell Line (T2-A) Cat. T8146 - Distant Pulmonary Metastasis Tumor Cell Line (T3-PA) Cat. T8147 - Distant Hepatic Metastasis Tumor Cell Line (T3-HA) Cat. T8148 - Highly Metastatic Distant Pulmonary Tumor Cell Line (T4-PA) Donor/background information provided for this product: Tumorigenic; obtained by subcutaneous hind quarter injection of parent cell line (GhrasT-NIH/3T3) in NIH Swiss mice and selection of cells from a hind quarter tumor..
Research relevance and current trends
- Cell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.
- Engineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.
- When metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.
Common research applications
- Cancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.
- Assay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.
- Side-by-side comparison of engineered versus parental background characteristics when relevant to the study design.
Changes in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.
Notes for experimental interpretation
- Morphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.
- Matched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.
Culture and product details
- Growth Conditions: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) . PriGrow III (TM003) + 10% FBS(Regular*) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Avoid excessive alkalinity in media and renew twice weekly. *Do not heat-inactivate
- Seeding Density (cells/cm²): 3,000 - 5,000
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
How should I handle live cells once I receive them?
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
Why are these cells classified as biosafety level II?
What is your warranty or return policy?
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
How many times can cells divide?
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
Ray, D.B., Merrill, G.A., Brenner, F.J., Lytle, L.S., Lam, T., McElhinney, A., Anders, J., Tauber, T.R., Lyker, J.K., Barcus, S., Leslie, K.H., Kramer, J.M., Rubenstein, E.M., Schanz, K.P., Parkhurst, A.J., Peck, M., Good, K., Granath, K.L., Cifra, N., Wantz Detweiler, J., Stevens, L., Albertson, R.A., Deir, R., Stewart, E., Wingard, K., Ricardson, M. R., Blizare, S. B., Gillespie, L. F., Kriley, C.F., Rzewnicki, D., and Jones, D.H., “T24 HRAS transformed NIH/3T3 mouse cells (GhrasT-NIH/3T3) in serial tumorigenic in vitro/in vivo passages give rise to increasingly aggressive tumorigenic cell lines T1-A and T2-A and metastatic cell lines T3-HA and T4-PA.” Experimental Cell Research, 340(1): (2016). 1-11. Open access: https://doi.org/10.1016/j.yexcr.2015.07.029
Towne, Camden M., Dudt, Jan F., and Ray, Durwood B., “Effect of Mansoa alliacea (Bignonaceae) leaf extract on embryonic and tumorigenic mouse cell lines.” Journal of Medicinal Plants Research Vol. 9(29). pp.799-805, August 3, 2015.
Scahill, Steven D., Sherman, Kelly Jean, Guidry, Jessie J., Walkowski, Whitney, Ray, Durwood B., Jones, David H., Gould, Harry J. III, and Paul, Dennis. In Vitro Characterization of a Novel Murine Model of Cancerous Progression, In preparation
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