| Field | Specification |
|---|---|
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Rat Primary Epidermal Keratinocytes are rat frozen primary cells from skin used for epidermal barrier biology, differentiation, and skin-response studies. These cells display adherent, cobble-stone growth characteristics.
Key elements and design rationale
- Biological source: Rat (R. norvegicus)
- Tissue origin: Skin
- Growth properties: Adherent, cobble-stone
- Format: Frozen
- Reported expression/markers: CK18, CK19
- Seeding guidance: 10,000 - 15,000 cells/cm²
- Biosafety level: II
Keratinocytes form the major cellular component of the epidermis and are widely used to study barrier formation, stratification-associated programs, inflammatory signaling, and wound-response biology.
Research relevance and current trends
- Primary keratinocyte cultures support studies of differentiation, barrier-associated gene expression, and inflammatory responses.
- Investigators often use co-culture or organotypic-adjacent systems to model skin microenvironment interactions.
- Donor-specific comparisons help capture variability in epithelial response and maturation state.
Common research applications
- Measure differentiation and barrier-associated marker changes under defined conditions.
- Study inflammatory, wound-response, or environmental stress signaling in skin-relevant systems.
- Use in co-culture assays with fibroblasts or immune cells to probe epithelial-stromal interactions.
Product-specific data supplied for this listing
- Growth Conditions: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM4960) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Seeding Density (cells/cm²): 10,000 - 15,000
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Marker panels should be interpreted together with morphology and functional readouts rather than as a standalone identity measure.
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
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