| Field | Specification |
|---|---|
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Rat Primary Renal Epithelial Cells are isolated from healthy rat kidney tissue and provide a versatile in vitro model for studying renal epithelial biology, transport functions, and kidney disease mechanisms. These cells retain characteristic epithelial morphology and marker expression, making them suitable for research in nephrotoxicity, inflammation, drug screening, and renal physiology studies. The product is supplied as frozen cells.
Key elements and design rationale
- Biological source: Rat (R. norvegicus)
- Tissue origin: Kidney
- Growth properties: Adherent, cobble-stone
- Format: Frozen
- Seeding guidance: 10,000 - 15,000 cells/cm²
- Biosafety level: II
- Culture context: PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Epithelial Cell Medium Kit (TM156) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C.
Epithelial cells form polarized barriers that regulate transport, secretion, and defense at tissue interfaces. Their phenotype is shaped by cell polarity, junctional organization, and tissue-specific differentiation cues.
Research relevance and current trends
- Primary epithelial models are widely used to examine barrier integrity, cytokine responses, and transport processes under physiologic conditions.
- Researchers often combine epithelial cells with stromal or immune components to model tissue microenvironments more faithfully.
- Donor-matched or disease-context epithelial cells help capture inter-individual heterogeneity in response assays.
Common research applications
- Measure barrier integrity, junctional organization, and polarized responses in vitro.
- Evaluate cytokine, pathogen, or drug responses in tissue-relevant epithelial cultures.
- Use in co-culture or air-liquid interface-adjacent workflows when epithelial context matters.
Product-specific data supplied for this listing
- Growth Conditions: PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Epithelial Cell Medium Kit (TM156) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C.
- Seeding Density (cells/cm²): 10,000 - 15,000
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
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