| Field | Specification |
|---|---|
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Rat Primary Retinal Microvascular Endothelial Cells are isolated from rat retinal microvasculature and exhibit characteristic cobblestone morphology and expression of endothelial-specific markers. These cells serve as a robust in vitro model for investigating retinal vascular biology, angiogenesis, blood–retinal barrier function, inflammation, and mechanisms of ocular diseases. The product is supplied as frozen cells.
Key elements and design rationale
- Biological source: Rat (R. norvegicus)
- Tissue origin: Eye
- Growth properties: Adherent, polygonal
- Format: Frozen
- Donor history: Normal tissue
- Biosafety level: II
- Culture context: PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. Endothelial Cell Medium Kit, Low Serum (TM155) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
Endothelial cells line the luminal surface of blood vessels and regulate barrier properties, hemostatic balance, immune cell trafficking, angiogenic signaling, and tissue-specific exchange processes.
Research relevance and current trends
- Primary endothelial models are used to study barrier integrity, inflammatory activation, and permeability changes in tissue-specific vascular beds.
- Co-culture systems with immune, stromal, or parenchymal cells are increasingly used to model microenvironment-dependent signaling.
- Researchers often compare donor, vessel-bed, or organ-specific phenotypes to capture biologically relevant heterogeneity.
Common research applications
- Assess barrier function, adhesion molecule regulation, or cytokine-driven endothelial activation in vitro.
- Model angiogenic responses, matrix interactions, and vessel-bed-specific signaling under defined culture conditions.
- Use in co-culture or transwell systems to study leukocyte transmigration and paracrine crosstalk.
Product-specific data supplied for this listing
- Growth Conditions: PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. Endothelial Cell Medium Kit, Low Serum (TM155) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Split Ratio: 1:2
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 8-10ml of pre-warmed, complete growth media. Centrifuge cells at 120xg for 5 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in 6ml of the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Change media the following day to remove non-adherent cells and replenish nutrients.
- Change media every 24-48 hours, and check cells daily under microscope to verify appropriate cell morphology. Change media every day when cells are >70% confluent. Pre-wash cells with 1X DPBS, No Ca, No Mg (CH110) 1-2 times whenever replacing media.
- Aspirate the culture media, and wash the adherent layer 1-2 times using 5ml sterile pipette with sterile 1X DPBS, No Ca, No Mg (CH110) to dislodge loosely attached cells and remove fraction. Remove and discard the wash solution from flask.
- Incubate cells with add 2ml of pre-warmed 0.05% Trypsin-EDTA for 3-5 minutes. As soon as cells detach (may require few firm gentle taps) add 8-10ml of complete culture media supplemented with 10% FBS to neutralize the trypsin.
- Plate cells in gelatin precoated flasks and incubate the cells at the recommended conditions.
- Change culture media the following day to remove non-adherent cells and replenish nutrients.
- Change media every 24-48 hours, and check cells daily under microscope to verify appropriate cell morphology. Change media every day when cells are >70% confluent. Pre-wash cells with 1X DPBS, No Ca, No Mg (CH110) 1-2 times whenever replacing media.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
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