RUNX3 Antibody, FITC conjugated

Overview

This polyclonal anti-RUNX3 antibody raised in Rabbit, conjugated with FITC. Designed to detect RUNX3 in Human for epigenetics and nuclear signaling research.

Key elements and design rationale

• Immunogen: Recombinant Human Runt-related transcription factor 3 protein (1-415AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
• FITC: Direct fluorescence (Ex490/Em525); protect from light; use antifade.

Biological background

RUNX3 (also: Acute myeloid leukemia 2 protein, Acute myeloid leukemia gene 2, AML 2, AML2, CBF alpha 3, CBF-alpha-3, CBFA 3, CBFA3, Core binding factor alpha 3 subunit, core binding factor, Core binding factor runt domain alpha subunit 3, Core binding factor subunit alpha 3, core-binding factor, Core-binding factor subunit alpha-3, Oncogene AML 2, Oncogene AML-2, PEA2 alpha C, PEA2-alpha C, PEBP2 alpha C, PEBP2-alpha C, Pebp2a3, PEBP2aC, Polyomavirus enhancer binding protein 2 alpha C subunit, Polyomavirus enhancer-binding protein 2 alpha C subunit, runt domain alpha subunit 3, runt related transcription factor 3, Runt-related transcription factor 3, RUNX 3, Runx3, RUNX3_HUMAN, SL3 3 enhancer factor 1 alpha C subunit, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core binding factor alpha C subunit, SL3/AKV core-binding factor alpha C subunit, Transcription factor AML2) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current RUNX3 annotation relevant to epigenetics and nuclear signaling.

Common research applications

• Refer to product documentation for confirmed application data.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Human; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.