SFPQ Antibody

Overview

This polyclonal anti-SFPQ antibody raised in Rabbit, tested in ELISA, WB. Designed to detect SFPQ in Human, Mouse for epigenetics and nuclear signaling research.

Key elements and design rationale

• Immunogen: Recombinant Human Splicing factor, proline- and glutamine-rich protein (499-598AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.

Biological background

SFPQ (also: 100 kDa DNA pairing protein, 100 kDa DNA-pairing protein, 100 kDa subunit, DNA binding p52/p100 complex 100 kDa subunit, DNA-binding p52/p100 complex, hPOMp100, Polypyrimidine tract binding protein associated splicing factor, Polypyrimidine tract-binding protein-associated-splicing factor, POMP100, PPP1R140, proline- and glutamine-rich, Protein phosphatase 1 regulatory subunit 140, PSF, PTB associated splicing factor, PTB-associated-splicing factor, Sfpq, SFPQ_HUMAN, Splicing factor, Splicing factor proline and glutamine rich, Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated), Splicing factor proline/glutamine rich) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current SFPQ annotation relevant to epigenetics and nuclear signaling.

Common research applications

• ELISA: Quantify soluble target; ensure samples within the linear detection range.
• WB: Confirms MW, distinguishes isoforms under denaturing conditions.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Human, Mouse; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (12556005)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.