SLC35A1 CRISPR Stable Knockout CHO-S Cell Line

What it means: A CRISPR Stable Knockout Cell Line is a mammalian cell line in which a specific gene has been permanently disrupted using CRISPR-Cas9 genome editing, with the modification stably integrated so it is maintained across cell passages.

Why it matters:

• Isogenic model: the CRISPR Stable Knockout Cell Line shares the same genetic background as its parental line, making it ideal for controlled loss-of-function comparisons.
• Stable vs. transient: unlike siRNA or transient transfection, stable knockout persists through repeated passaging without re-transfection, enabling long-term assays and clonal expansion.
• CRISPR-Cas9 mechanism: edits are typically frameshift-inducing INDELs confirmed by Sanger Sequencing, resulting in non-functional protein output from the targeted allele(s).
• Selection: puromycin or equivalent antibiotic resistance confirms stable integration — maintain selection pressure after the first passage post-thaw.

How to interpret here: This product is a CRISPR Stable Knockout Cell Line, meaning the gene disruption is permanent and heritable. Always culture with the recommended selection antibiotic (after first passage) to maintain knockout fidelity over time. Validate knockout status by Western blot or Sanger Sequencing before critical experiments.

What it means: Frozen indicates that this cell line is supplied cryopreserved — typically 1×10⁶ viable cells in 1.0 ml cryoprotective medium — and shipped on dry ice for immediate transfer to liquid nitrogen vapor phase storage.

Why it matters:

• Immediate handling: Frozen cells must be transferred to liquid nitrogen vapor phase (below −130°C) upon receipt; do not store at −70°C, as this causes gradual loss of viability.
• Recovery time: allow one full passage post-thaw before starting experiments — cells need time to recover from freeze-thaw stress and re-establish normal physiology.
• Selection drug timing: add the selection antibiotic only after the first passage post-thaw; applying selection too early risks killing recovering cells.

How to interpret here: This product ships as Frozen cryopreserved cells. Rapid thawing in a 37°C water bath (≤2 minutes), immediate dilution in pre-warmed complete medium, and centrifugation to remove cryoprotectant are essential first steps. Viability is warranted for 30 days post-shipment when handled per abm guidelines.

Tip: Do not refreeze thawed cells — once thawed, proceed directly to culture initiation.