TAGLN Antibody Pair

Overview

This is a matched capture and detection antibody pair designed for sandwich ELISA quantification of Transgelin in Human,Rat,Mouse,Guinea pig,Bovine,Horse,Pig,Dog,Rabbit,Goat,Sheep,Duck. The pair consists of a capture antibody (unconjugated) and a detection antibody (biotin-conjugated), both raised in Capture: Rabbit | Detection: Rabbit, enabling a complete sandwich format without the need for additional antibody sourcing.

Key elements and design rationale

• Clonality: Capture: Polyclonal | Detection: Polyclonal — each component is independently validated for capture and detection roles in the sandwich format.
• Conjugation format: Capture: Unconjugated | Detection: Biotin conjugated — the detection antibody carries the biotin label for streptavidin-amplified signal generation; the capture antibody is unconjugated for plate coating.
• Isotype: Capture: IgG | Detection: IgG — matching isotype across capture and detection components reduces non-specific background from secondary reagents.
• Application notes: We recommend using the capture antibody at a concentration of 0.8ug/ml and the detection antibody at a concentration of 0.25ug/ml.Optimal dilutions should be determined experimentally by the researcher.

Biological background

Transgelin (also referred to as 22 kDa actin-binding protein,Protein WS3-10,Smooth muscle protein 22-alpha,SM22-alpha) is a protein target of interest in Human,Rat,Mouse,Guinea pig,Bovine,Horse,Pig,Dog,Rabbit,Goat,Sheep,Duck systems. Researchers studying Transgelin typically rely on quantitative sandwich ELISA to measure protein levels in serum, plasma, cell culture supernatants, or tissue homogenates. Consult UniProt, NCBI Gene, and primary literature for current annotation of Transgelin expression, function, and disease relevance.

Common research applications

• Sandwich ELISA quantification: The matched pair is optimized for standard sandwich ELISA formats. The capture antibody is coated to the plate surface; after sample incubation, the biotinylated detection antibody is added, followed by streptavidin-HRP and colorimetric substrate for quantification.
• Sample types: Typically validated for serum, plasma, and cell culture supernatants. Confirm sample type compatibility with the product datasheet before use in other matrices.

Notes for experimental design

• Antibody concentrations: We recommend using the capture antibody at a concentration of 0.8ug/ml and the detection antibody at a concentration of 0.25ug/ml.Optimal dilutions should be determined experimentally by the researcher.
• Standard curve: Use recombinant Transgelin protein as a standard for absolute quantification; confirm standard purity and activity before use.
• Cross-reactivity: Species reactivity is reported for Human,Rat,Mouse,Guinea pig,Bovine,Horse,Pig,Dog,Rabbit,Goat,Sheep,Duck. Use in other species matrices requires empirical confirmation of antibody–antigen binding.
• Streptavidin-HRP: Select a high-quality streptavidin-HRP conjugate and optimize its concentration to minimize background while maintaining signal sensitivity.

• Datasheet (11117206)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.