| Field | Specification |
|---|---|
| Alternative Names | Thiaminase-1; EC 2.5.1.2; Thiaminase I; Thiamine pyridinylase |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Paenibacillus thiaminolyticus Thiaminase-1 protein (30-409AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This polyclonal anti-Thiaminase-1 antibody raised in Rabbit, tested in ELISA, WB. Designed to detect Thiaminase-1 in Paenibacillus thiaminolyticus for others research.
Key elements and design rationale
- Immunogen: Recombinant Paenibacillus thiaminolyticus Thiaminase-1 protein (30-409AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
Biological background
Thiaminase-1 (also: Thiaminase-1, EC 2.5.1.2, Thiaminase I, Thiamine pyridinylase) — studied in Paenibacillus thiaminolyticus systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current Thiaminase-1 annotation relevant to others.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
- WB: Confirms MW, distinguishes isoforms under denaturing conditions.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Paenibacillus thiaminolyticus; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
Thiaminase-1 is a protein studied in Paenibacillus thiaminolyticus biology. This polyclonal antibody raised in Rabbit detects Thiaminase-1 in ELISA, WB applications, IgG isotype.
Reported reactive against Paenibacillus thiaminolyticus. Immunogen from Paenibacillus thiaminolyticus. Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA, WB. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
Non-conjugated IgG; requires anti-rabbit-IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin).
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing Thiaminase-1; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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