Protein Quality Control — Antibodies

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2520 products
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Western Blot
Positive WB detected in:MCF-7 whole cell lysate(30µg) , K562 whole cell lysate(30µg) , NIH/3T3 whole cell lysate(30µg) 
All lanes: Hsp70 4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 94 kDa
Observed band size: 120 kDa
Exposure time:1min20s
Target: HSPA4
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunofluorescence, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA062281A0HU diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Target: HSPB8
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Immunofluorescence staining of MCF-7 cell with CSB-RA036685A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
Target: UBB
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunofluorescence, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: A549 whole cell lysate(30µg) , HeLa whole cell lysate(30µg) , HepG2 whole cell lysate(30µg) , HEK293 whole cell lysate(30µg) , K562 whole cell lysate(30µg) , SH-SY5Y whole cell lysate(30µg) , NIH/3T3 whole cell lysate(30µg) 
All lanes: UBE2I antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 3min
Target: UBE2I
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA012285A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Target: UBB/UBC
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry, Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: U-251MG whole cell lysate(20µg) , HepG2 whole cell lysate(20µg) , Hela whole cell lysate(20µg) , colo205 whole cell lysate(20µg) , A549 whole cell lysate(20µg) , PC-3 whole cell lysate(20µg) 
All lanes: HSPA5 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: 2min
Target: HSPA5
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: A549 whole cell lysate(20µg) , PC-3 whole cell lysate(20µg) , U-251MG whole cell lysate(20µg) , MCF-7 whole cell lysate(20µg) , K562 whole cell lysate(20µg) , HepG2 whole cell lysate(20µg) , HeLa whole cell lysate(20µg) , HEK293 whole cell lysate(20µg) , NIH/3T3 whole cell lysate(20µg) 
All lanes: HSPA8 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time:10s
Target: HSPA8
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Overlay Peak curve showing MCF7 cells stained with CSB-RA316735MA1HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10 6 cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C.
Target: UBC
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA025440MA1HU diluted at 1:300 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration,  antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Target: UBE2C
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA010833MA1HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration,  antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Target: HSPB1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA644510A0HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.74% DAB.
Target: HSP90AB1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA980583A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.57% DAB.
Target: MDM2
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: HepG2 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate
All lanes: HSP90AB1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84 kDa
Observed band size: 80-90 kDa
Target: HSP90AB1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate
All lanes: RNF20 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 114 kDa
Observed band size: 114 kDa
Target: RNF20
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA898115A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Target: CUL3
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA847116A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Target: HSPA5
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, Mouse brain tissue
All lanes: HSPD1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62, 18 kDa
Observed band size: 60 kDa
Target: HSPD1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
From $210.00
Regular price From $210.00
In Stock at Manufacturer
HSPA8 Antibody – Figure 1 of 1
Target: HSPA8
Primary Antibody ELISA, Western Blot
From $210.00
Regular price From $210.00
In Stock at Manufacturer
HSP90AA1/HSP90AB1 Antibody – Figure 1 of 3
Target: HSP90AA1/HSP90AB1
Primary Antibody ELISA, Western Blot, Flow Cytometry, Immunoprecipitation
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue
All lanes: Hsp90 alpha antibody at 0.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85, 99 KDa
Observed band size: 85 KDa
Target: HSP90AA1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in A549 whole cell lysate(treated with EGF or not) 
All lanes Phospho-HSPB1 antibody at 1.5μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 27 KDa
Observed band size: 27 KDa
Target: HSPB1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in Hela whole cell lysate, HepG2 whole cell lysate(treated with Calyculin A or EGF) 
All lanes Phospho-HSPB1 antibody at 0.73μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 27 KDa
Observed band size: 27 KDa
Target: HSPB1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: Hsp90 alpha antibody at 1.9μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85, 99 KDa
Observed band size: 90 KDa
Target: HSP90AA1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA011087A1HU diluted at 1:87.5 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Target: HSP90AA1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry, Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Hela whole cell lysate, PC3 whole cell lysate, 293T whole cell lysate, LO2 whole cell lysate, HT-29 whole cell lysate, K562 whole cell lysate
All lanes: Hsp27 antibody at 0.62μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 23 KDa
Observed band size: 27 KDa
Target: HSPB1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
From $210.00
Regular price From $210.00
In Stock at Manufacturer
No image
Target: RNF122
Antibodies, Primary Antibodies Western Blot
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: RNF122
Antibodies, Primary Antibodies ELISA
From $166.00
Regular price From $166.00
In Stock at Manufacturer
IHC image of CSB-PA019825LA01HU diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Target: RNF122
Antibodies, Primary Antibodies ELISA, Immunohistochemistry, Immunofluorescence
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: RNF122
Antibodies, Primary Antibodies ELISA
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: UBB
Antibodies, Primary Antibodies Western Blot
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: UBB
Antibodies, Primary Antibodies ELISA
From $166.00
Regular price From $166.00
In Stock at Manufacturer
IHC image of CSB-PA04837A0Rb diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
Target: UBB
Antibodies, Primary Antibodies ELISA, Immunohistochemistry
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: UBB
Antibodies, Primary Antibodies ELISA
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: HSPA1A
Antibodies, Primary Antibodies Western Blot
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: HSPA1A
Antibodies, Primary Antibodies ELISA
From $166.00
Regular price From $166.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: A549 whole cell lysate, COLO320 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate
All lanes: HSPA1A antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 71, 64 kDa
Observed band size: 71 kDa
Target: HSPA1A
Antibodies, Primary Antibodies ELISA, Western Blot, Immunohistochemistry
From $166.00
Regular price From $166.00
In Stock at Manufacturer

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