DENARASE® toolkit - Enzymatic DNA Removal in Bioprocessing

DENARASE® is a Serratia marcescens-derived endonuclease from c-LEcta, engineered for efficient nucleic acid clearance across GMP biomanufacturing workflows — including viral vector production, vaccine manufacturing, and downstream bioprocessing. The portfolio covers the full salt spectrum: the wild-type DENARASE® delivers peak performance at physiological salt conditions (0–150 mM, pH 7.4–9), while DENARASE® High Salt extends activity to 0–500 mM salt — enabling higher viral vector yields in salt-supplemented processes. Both variants are produced free of animal-derived materials, antibiotics, and endotoxin-forming hosts, with R&D and GMP grades offering equivalent technical performance for seamless process scale-up. The companion DENARASE® ELISA Kit provides monoclonal antibody-based quantification of residual endonuclease — covering both wild-type and High Salt variants — for unmatched accuracy and reproducibility in clearance validation.

3Products
3 products
Image ProductProduct Type ApplicationPrice Status ACTION
DENARASE®- Precision Nuclease for Cleaner Bioprocessing
Best Seller Featured GMP Available Peer-Reviewed Free Sample
DENARASE®- Precision Nuclease for Cleaner Bioprocessing
Enzyme, Endonuclease —
From $599.50
From $599.50
In Stock
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DENARASE® High Salt – Salt-Tolerant Nuclease for High-Efficiency DNA Removal
New Featured GMP Available Peer-Reviewed Free Sample
DENARASE® High Salt – Salt-Tolerant Nuclease for High-Efficiency DNA Removal
Enzyme, Endonuclease —
From $363.00
From $363.00
In Stock
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DENARASE® ELISA kit The One-for-all Serratia marcescens ELISA Kit
Best Seller Featured Peer-Reviewed Bulk Pricing
DENARASE® ELISA kit The One-for-all Serratia marcescens ELISA Kit
ELISA Kits ELISA
From $78.00
From $78.00
In stock
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DENARASE® Collection — eBioHippo
Featured Supplier c-LEcta GmbH

DENARASE® Toolkit
Enzymatic DNA Clearance in Bioprocessing

DENARASE® enzymes efficiently remove nucleic acids in GMP manufacturing processes. The product line includes the wild-type DENARASE® at physiological salt conditions and the engineered DENARASE® High Salt for broader salt tolerance — together covering the full salt spectrum for optimal flexibility. The companion DENARASE® ELISA Kit enables monoclonal antibody-based quantification of residual endonuclease for clearance validation.

≥98%
Purity
SDS-PAGE
>250
U/µl
Activity
0–500
mM Salt
Coverage
≥98% Purity EU GMP Compliant ISO 9001 R&D Grade Cleaves ss/ds DNA & RNA No Animal-Origin Materials Endotoxin-Free Host R&D → GMP Scale-Up
Product Line

DENARASE® Toolkit — Three Products, Complete Coverage

R&D and GMP grades are technically equivalent, enabling seamless transition from process development to commercial manufacturing. The ELISA Kit covers both wild-type and High Salt variants in a single assay.

10% Off — R&D Grade
DENARASE®
Wild-type · Physiological Salt
  • Peak performance at physiological salt levels
  • High activity on all DNA/RNA forms (ss, ds, circular, supercoiled)
  • Purity ≥98% · Activity >250 U/µl
  • R&D (ISO 9001) & GMP (EU GMP) grades — equivalent performance
  • Most economical choice for low-to-physiological salt processes
Salt range
0–150 mM
pH range
7.4–9
Purity
≥98%
Activity
>250 U/µl
R&D grade · One order: DENARASE10
GMP grade excluded from promotion
View R&D Grade → View GMP Grade
10% Off — R&D Grade
DENARASE® High Salt
Engineered · Extended Salt Range
  • Designed for improved salt & pH tolerance (0–500 mM)
  • Enables higher viral vector yields via salt-supplemented lysis
  • Purity ≥98% · Activity >250 U/µl — equivalent to wild-type
  • R&D (ISO 9001) & GMP (EU GMP) grades available
  • Greatest flexibility & cost-efficiency for high-salt processes
Salt range
0–500 mM
pH range
7.4–9
Purity
≥98%
Activity
>250 U/µl
R&D grade · One order: DENARASE10
GMP grade excluded from promotion
View R&D Grade → View GMP Grade
10% Off — New Customers
DENARASE® ELISA Kit
One-for-all Serratia marcescens
  • Quantifies residual DENARASE® & DENARASE® High Salt
  • Monoclonal antibody — unmatched accuracy & reproducibility
  • 50× higher sensitivity than competitor kits
  • Pre-coated 96-well plate · all ready-to-use reagents included
  • Results in half the time of comparable kits
Cat.#
BHE16500002
Format
96-well
Price
$4,195
Stock
In Stock
New customers · First order: ELISA10
View ELISA Kit →
Salt Spectrum Coverage

Covering the Whole Salt Spectrum

The DENARASE® portfolio enables efficient DNA removal across a broad salt spectrum and different pH levels. At low-to-physiological salt conditions, wild-type DENARASE® delivers peak performance and remains the most economical solution. For processes that benefit from salt addition, DENARASE® High Salt offers the greatest flexibility and cost-efficiency.

0 mM100 mM200 mM300 mM400 mM500 mM
DENARASE®
0–150 mM · pH 7.4–9
150 mM
DENARASE® High Salt
0–500 mM · pH 7.4–9
DENARASE® — optimal below 150 mM
DENARASE® High Salt — full 0–500 mM range
150 mM — recommended switchover point
Parameter DENARASE® DENARASE® High Salt
Recommended salt 0–150 mM 150–500 mM
Full active range 0–150 mM 0–500 mM
pH range 7.4–9 7.4–9
Purity ≥98% (SDS-PAGE) ≥98% (SDS-PAGE)
Activity >250 U/µl >250 U/µl
Grades R&D (ISO 9001) · GMP (EU GMP) R&D (ISO 9001) · GMP (EU GMP)
Best for Economical · low/physiological salt High-salt lysis · improved vector yield
Why DENARASE®

Key Features Across the Toolkit

EU GMP Compliant Manufacturing
Produced under EU GMP regulations. R&D and GMP grades offer equivalent performance for seamless process scale-up.
No Animal-Origin Materials
Free of animal-derived materials, antibiotics, and Triton X-100. No TSE/BSE risk. Gram-positive Bacillus sp. host.
High Activity on All Nucleic Acids
Cleaves ss/ds DNA and RNA — linear, circular, and supercoiled. Produces fragments of ~3–5 bp.
Endotoxin-Free Expression Host
Gram-positive Bacillus sp. host eliminates endotoxin risk inherent to gram-negative expression systems.
Seamless R&D → GMP Transition
Switch grades without revalidation. Change Guidance Documents available for regulatory submissions.
One-for-All ELISA Validation
Single mAb-based ELISA detects both variants. 50× higher sensitivity. Results in half the time of competitors.
Current Promotions

10% Off One Order — Two Active Offers

10% Off
DENARASE® R&D Grade
Wild-type & High Salt
One order · R&D grade only · GMP grade excluded
Promo Code DENARASE10
One order per customer  ·  R&D grade DENARASE® only  ·  GMP grade excluded  ·  Cannot be combined  ·  Net price ex. VAT & shipping
10% Off
DENARASE® ELISA Detection Kit
New customers · first order · applied at cart checkout
BHE16500002
Promo Code ELISA10
Valid for new customers only  ·  One-time use per customer  ·  Applies to Cat.# BHE16500002 only  ·  Cannot be combined  ·  Net price ex. VAT & shipping
Scientific specifications sourced from c-LEcta GmbH. DENARASE® is a registered trademark of c-LEcta GmbH. Enzyme hydrolyzes phosphodiester bonds, producing fragments of ~3–5 bp. Active on all nucleic acid forms (ss/ds, linear, circular, supercoiled DNA and RNA). Production host: gram-positive Bacillus sp. — no TSE/BSE risk, no Triton X-100, no animal-origin inputs. R&D grade: ISO 9001. GMP grade: EU GMP compliant. Promotions: 10% off one order via code DENARASE10 (R&D grade endonuclease only; GMP excluded) and ELISA10 (Cat.# BHE16500002 only) — new customers, one-time use, cannot be combined. Net price ex. VAT and shipping. Not for therapeutic or clinical use.

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