BrainVTA offers tailor-made AAVs for the customers and presents a wide variety of options to choose from. All customized services undergo stringent control to guarantee the quality of the viral particles you receive each time.
● The packaging capacity is~4.7 kb from ITR to ITR for single stranded AAV and ~2.2 kb from ITR to ITR for self-complementary AAV.
● Viral particles are purified by ultracentrifugation on an iodixanol step gradient.
● Quality control assays include qPCR to quantify viral titers and silver stain to determine viral titer and purity after purification.
● Serotypes include 1, 2, 5, 6, 8, 9, rh10, DJ, DJ8, PHP.B, PHP.eB, PHP.S, retro, anc80. Novel peptide modified serotypes can also be produced on a case-by-case basis. For information on Serotype and Tissue Specificity, please visit introduction toAAV Services.
● Here are some examples of different types of custom-made AAV vectors that have been produced successfully:
-Cre / FlpO
-Tracing virus helper
AAV specific infection in some organs and tissues.
Large Scale Custom-Made AAVs Production
BrainVTA has established a serum-free cell suspension culture system, through which we offer large scale production with serum free, antibiotic free, highly purified and highly potent products.
Find out our custom-Made AAV service following the guide below:
● Submit your needs to us by clicking theSend Requestbutton or email us at[email protected] and our experienced scientists will design your vector for free.
● Or, for any customers who decides to take use of viruses as nucleotide sequence delivery tools, our scientists will work with you step-by-step from design of study to viral manufacturing to facilitate your research.
*The virus titer is determined by the viral genome copy number by real-time quantitative PCR using primers targeting the inserted DNA sequences such as wpre, cmv and GFP instead of ITRs to avoid false high titers.
** An additional fee is charged for plasmid construction service. For detailed price,contact us. **Shipping Fees may range from $60 to $100, depending on locations. Please click the Send Requestbutton directly if none of the above serves your purpose. Our highly experienced scientists will always be ready to work you through the jungle.
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