| Field | Specification |
|---|---|
| Species |
- Antigen expression: CD10+, CD19+, CD20+, CD21(+), CD22+, CD23-, CD24-, CD32+, CD37+, CD38+, CD39-, CD40+, CD54+, CD72+, CD73-, CD75+, CD77+, CD81, CD82+, CD83+, CD84+, CD86+
- Karyotype: 46, hypodiploid
- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with 20% FBS, 10 mM HEPES
- subculturing: Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 105 cells/ml and keep the cell concentration within the range of 3 x 105 to 1 x 106 cells/ml for optimal growth.
- seedingDensity: 3 x 105 cells/ml
- fluidRenewal: Every 3 to 5 days
- postThawRecovery: Allow the cells to recover from the freezing process for at least 48 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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