| Field | Specification |
|---|---|
| Species |
Jurkat cells, which originated from the peripheral blood of a 14-year-old with T-cell acute lymphoblastic leukemia (T-ALL), are a well-known human T lymphocyte cell line commonly used in cell biology studies, particularly in cancer research and immune system disorder investigations. These cells play a crucial role in understanding various cellular processes, including cell death mechanisms, autophagy activity, and cytoplasmic transcription factors.
Jurkat cells are commonly used in HIV research due to their expression of the CD4 receptor on their cell membrane. The CD4 receptor is a primary receptor that HIV uses to enter host cells. Because Jurkat cells express this receptor, they can be infected by HIV, making them a useful model for studying HIV's interactions with human T cells, which are a major target of the virus in the human body. The utilization of Jurkat cells in HIV activation and the HIV infection life cycle studies has significantly contributed to understanding the virus's interactions with human cells and has been instrumental in identifying potential targets for antiretroviral therapies.
Jurkat cells further play a pivotal role in biomedical research, particularly in the evaluation of cytotoxicity and cell viability assays. This makes them indispensable for testing the effectiveness of potential cancer therapies and agents that modulate the immune response. By employing Jurkat cells, scientists can meticulously analyze the effects of cytotoxic compounds on the integrity and function of the cell membrane, including aspects related to cell membrane permeability and their transport properties.
Furthermore, the presence of mutations in the Lck gene within Jurkat cells, which leads to sustained activation of T-cells, provides a unique model for in-depth studies of T-cell activation and signaling pathways. This is essential for understanding the complex processes of lymphocyte activation, which encompasses the cell cycle, cellular growth, and differentiation. Such insights are crucial for developing strategies to modulate immune responses in various diseases.
The creation of a specific Jurkat cell derivative, known as Jurkat E6.1, has significantly advanced our comprehension of cellular mechanisms. This derivative offers a refined tool for probing into the nuanced behaviors of cell membranes and the physiological responses of individual cells under experimental conditions. Through the use of Jurkat E6.1 cells, researchers have been able to shed light on fundamental cellular processes and their implications for health and disease.
In summary, Jurkat cells serve as invaluable tools in a wide range of research areas, from cancer biology to HIV infection studies, offering insights into cell biology, immune system function, and potential therapeutic interventions.
—
- Antigen expression: Jurkat cells express T-cell receptor (TCR) and CD3 proteins. They also express CD4 and CD8 co-receptors, which aids in identifying them as helper or cytotoxic T cells.
- Mutational profile: The Jurkat cell line has been reported to have genetic mutations primarily impacting three core pathways: TCR signaling, genome stability, and O-linked glycosylation. In TCR signaling, mutations in PTEN, INPP5D, CTLA4, and SYK disrupt normal cellular responses to T-cell receptor activation, potentially affecting proliferation and survival. Genome stability is compromised by mutations in TP53, BAX, and MSH2, leading to impaired DNA repair mechanisms and an increased susceptibility to tumorigenesis. Additionally, a mutation in C1GALT1C1 disrupts O-linked glycosylation processes, resulting in the expression of truncated O-glycans [1]. Additionally, the Jurkat cells have a point mutation in the Lck gene, which encodes a protein necessary for T-cell activation, causing T cells to be constitutively activated. References: 1. Gioia, L., Siddique, A., Head, S. R., Salomon, D. R., & Su, A. I. (2018). A genome-wide survey of mutations in the Jurkat cell line. BMC genomics, 19, 1-13.
- Karyotype: The Jurkat cell line is hypotetraploid with a flat modal karyotype of 46 chromosomes and 7.8% polyploidy.
- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with 10% heat-inactivated FBS
- doublingTime: 26 hours
- subculturing: Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 105 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets — use code SIRNA10
20% Off Transmembrane Proteins — use code TM20
$50 Off All ELISA Kits — auto-applied at checkout (code ELISA50)
50% Off Lab Consumables + Free Shipping on orders $500+
15% Off Proteins from Trusted Suppliers — use code PROTEIN15
$99 Pipette Filler Promotion Package (reg. $236)
Save 10% on your first order of any R&D grade DENARASE® with code DENARASE10 at checkout.
