| Field | Specification |
|---|---|
| Species |
MH-S is a murine alveolar macrophage cell line derived from adult mice. These cells are widely used in immunological research due to their robust phagocytic activity and their ability to produce a variety of cytokines in response to pathogenic stimuli. As an alveolar macrophage model, MH-S cells are particularly valuable in studying pulmonary immune responses, lung inflammation, and respiratory infections. Their ability to mimic the behavior of primary alveolar macrophages makes them an indispensable tool for understanding the mechanisms of host defense in the respiratory tract.
MH-S cells are also instrumental in the study of macrophage biology and function. They are employed to investigate macrophage activation, differentiation, and the signaling pathways involved in immune responses. Researchers utilize this cell line to explore the interactions between macrophages and pathogens, including bacteria, viruses, and fungi. Additionally, MH-S cells serve as a model to examine the effects of various pharmacological agents on macrophage activity, offering insights into potential therapeutic approaches for respiratory diseases.
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- Protein expression: interleukin 1 (IL-1)
- Antigen expression: CD11b (Mac-1), Class II antigens (I-A), T antigen
- Viruses: Transformant: Simian virus (SV40)
- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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