| Field | Specification |
|---|---|
| Species |
The B16-F0 cell line is a murine melanoma cell line derived from the B16 mouse melanoma. This cell line is widely used in cancer research due to its high metastatic potential and ability to form tumors when injected into syngeneic mice. B16-F0 cells are particularly useful for studying the molecular mechanisms underlying melanoma progression and metastasis, as well as for testing the efficacy of anti-cancer drugs and therapeutic interventions in preclinical models. Notably, the B16-F0 cell line is the parent cell line from which other variants, such as B16-F1, B16-F10, and B16-BL6, have been derived through selective procedures aimed at enhancing specific metastatic properties.
B16-F0 cells exhibit a typical epithelial morphology and grow adherently in culture. They are known to express various melanoma-associated antigens, making them a valuable tool for immunological studies and for developing melanoma vaccines. Additionally, these cells are often employed in studies involving gene expression, signaling pathways, and the tumor microenvironment. Researchers utilize B16-F0 cells to explore the interactions between melanoma cells and the immune system, particularly focusing on immune evasion and suppression mechanisms. The characterization of B16-F0 and its derived lines provides a comprehensive framework for understanding the invasive and metastatic behaviors of melanoma, with B16-F1, B16-F10, and B16-BL6 each representing stages of increasing metastatic and invasive activity, thereby serving as critical models in the study of cancer progression and therapeutic response.
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- Tumorigenic: Yes, in syngeneic mice
- Products: Melanin
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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